Antimicrobial activity and cytotoxicity towards cancer cells of Melaleuca alternifolia (tea tree) oil
- 447 Downloads
Antimicrobial and in vitro anti-tumor activities of Melaleuca alternifolia (tea tree) oil (TTO) were analyzed. The results indicated that TTO possessed significant antimicrobial activity towards all tested microorganisms except Aspergillus niger. The minimum inhibitory concentration (MIC) of TTO towards Staphylococcus epidermidi, Bacillus subtilis, Escherichia coli and Candida albicans was 0.25% (v/v). Time kinetic experiments showed that Staphylococcus epidermidi, Escherichia coli and Candida albicans were completely killed after exposure to TTO at 2× MIC for 1, 4 and 8 h, repectively. TTO also exhibited strong cytotoxicity towards human lung cancer cell line (A549), human breast cancer cell line (MCF-7) and human prostate cancer cell line (PC-3). The IC50 values obtained were 0.012% (v/v), 0.031% (v/v) and 0.037% (v/v), respectively. Flow cytometric analysis demonstrated that TTO restrained the cell cycle progression of PC-3 and MCF-7 cell lines at the G0/G1 phase and A549 cell at the S phase.
KeywordsAntimicrobial activity Cell cycle Cytotoxicity to tumor cells Melaleuca alternifolia (Myrtaceae) Tea tree oil
The authors gratefully acknowledge the financial supports from the National Natural Science Foundation of China (30770231), National Key Technology R & D Program (2006BAD18B0401), the Agriculture Commercialization of Research Findings Foundation (05EFN21700432), Innovative Program for Importation of International Advanced Agricultural Science and Technology, Nation Forestry Bureau (2006-4-75), and Research Foundation for Science and Technology Innovation Talents of Harbin (2006RFXXS001).
- 2.Kotan R, Kordali S, Cakir A (2007) Z Naturforsch C 62:507–513Google Scholar
- 8.Carson CF, Reily TV (1995) J Appl Bacteriol 78:264–269Google Scholar
- 10.Woollard AC, Tatham KC, Barker S (2007) J Wound Care 16:255–257Google Scholar
- 12.Schnitzler P, Schön K, Reichling J (2001) Pharmazie 56:343–347Google Scholar
- 13.Hayes AJ, Leach DN, Markham JL (1997) J Essent Oil Res 9:575–582Google Scholar
- 15.Carson CF, Hammer KA, Riley TV (2006) Clin Microbiol Rev 1950–1962Google Scholar
- 23.Mondello F, Gentile E, Girolamo A, Salvatore G (2003) Piante Med 2:185–193Google Scholar
- 28.Uribe S, Ramirez J, Peña A (1985) J Bacteriol 161:1195–1200Google Scholar
- 30.Moreiraa MR, Ponceb AG, del Vallea CE, Roura SI (2005) Food Sci Technol 38:565–570Google Scholar
- 33.Hammer KA, Carson CF, Riley TV, Nielsen JB (2006) Food Chem. Toxicol 44:616–625Google Scholar