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Detection of genetically modified rice: collaborative validation study of a construct-specific real-time PCR method for detection of transgenic Bt rice


A collaborative trial study has been conducted for validation of an extraction method and a subsequent real-time PCR for detection of a transgenic Bt rice line (‘Bt63’) in rice products originating from China. A total of 17 laboratories participated in the study and each laboratory received 16 coded samples comprising of rice grain flours, rice noodle flours and plasmid DNAs. Of the accepted results all Bt63-positive rice grain samples (0.1 or 0.05% w/w) and all rice noodle samples prepared from marketed rice products were detected correctly. The result demonstrates that ‘Bt63’ rice is detectable even at low relative mass concentrations of 0.05%. The absolute LOD determined with plasmid DNA samples showed to be at least five copies of the ‘Bt63’ target sequence. The data provided in this study show that the method is fit-for-purpose to inspect Chinese rice products for the presence of EU-unauthorised rice lines carrying the ‘Bt63’ construct.

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The authors are very grateful to Christine Degner, Katja Trübner and Dr. Gunther Ruick for their help and constant support during this study. We thank Dr. Frank Gebhardt, Dr. Ralf Reiting and Dr. Norbert Hess for providing positive sample materials and Dr. Dirk Engelbert for his help to conduct the study. We would like to thank Dr. Joachim Bendiek for carefully reading the manuscript and his helpful comments. The authors are also grateful to all participants of the collaborative trial: Norbert Graf, Landesuntersuchungsamt Rheinland-Pfalz, Institut für Lebensmittelchemie (Trier), Anette Hilpert, Thüringer Landesamt für Lebensmittel und Verbraucherschutz (Jena), Anja Palisch, Congen GmbH (Berlin), Daniel Suter, Bundesamt für Gesundheit, Abt. Lebensmittelwissenschaft (Bern/Schweiz), Margit Hagen, Chemisches und Veterinäruntersuchungsamt Ostwestfalen-Lippe (Detmold), Birgit Ehrentreich, Landesveterinär- u. Lebensmitteluntersuchungsamt Mecklenburg-Vorpommern (Rostock), Claudia Brünen-Nieweler, Chemisches Landes- und Staatliches Veterinäruntersuchungsamt (Münster), Antje Dietz-Pfeilstetter, Biologische Bundesanstalt für Land- und Forstwirtschaft (Braunschweig), Norbert Hess, Institut für Hygiene und Umwelt (Hamburg), Rupert Hochegger, Agentur für Gesundheit und Ernährungssicherheit—AGES (Wien/Österreich), Hermann Rüggeberg, Impetus GmbH (Bremerhaven), Karl Woll, Landesamt für Soziales, Gesundheit und Verbraucherschutz (Saarbrücken), Hans-Ulrich Waiblinger, Chemisches und Veterinäruntersuchungsamt (Freiburg), Ralf Reiting, Landesbetrieb Hessisches Landeslabor (Kassel), Peter Hübert, Landeslabor Schleswig-Holstein (Neumünster), Gabi Mücher, GEN-IAL GmbH (Troisdorf), Anne Nemeth, Eurofins/GeneScan (Freiburg).

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Correspondence to Lutz Grohmann.

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Grohmann, L., Mäde, D. Detection of genetically modified rice: collaborative validation study of a construct-specific real-time PCR method for detection of transgenic Bt rice. Eur Food Res Technol 228, 497–500 (2009).

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  • Genetically modified rice
  • Detection method
  • Real-time PCR
  • Collaborative trial
  • Bt63