Abstract
The antioxidant activity (free radical scavenging ability and lipid oxidation inhibitory activity) and the activity in cell culture [influence on HT-29 human colon cancer cell viability and nuclear factor kappa B (NF-κB) activation] of natural fruit juices from red grape, strawberry, cherry and sour cherry were investigated in vitro. Analysis of polyphenolic composition of fruit juices showed the dominant presence of anthocyanins (cyanidin-3-glucoside, cyanidin-3-rutinoside, peonidin-3-rutinoside, pelargonidin-3-glucoside), anthocyanidins (procyanidins B1, B2 and B3), flavonoids (glucosides of myricetin, quercetin and kaempferol), and phenolic acids (neochlorogenic acid, chlorogenic acid, ellagic acid, p-coumaroylquinic acid) in juices. DPPH test and β-carotene bleaching method indicated that grape, strawberry, cherry and sour cherry natural juices (at the concentration of 2.5%) showed very strong free radical scavenging (75–92%) and antioxidant activity (71–94%). In addition, at the concentration of 0.01% they showed complete inhibition of copper-induced oxidation of human LDL (low density lipoprotein). In contrast, selected fruit juices did not show cytotoxic effects on HT-29 cells and NF-κB inhibitory activity (at the concentrations of 0.5 and 2%). Results of this study showed no correlation between the antioxidant activity of natural fruit juices from red grape, strawberry, cherry and sour cherry and their effect on HT-29 cell viability and NF-κB inhibitory activity.
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Abbreviations
- DPPH:
-
2,2′-Diphenyl-1-picrylhydrazyl
- EGFP:
-
Enhanced green fluorescent protein
- SDS:
-
Sodium dodecyl sulfate
- TNF:
-
Tumor necrosis factor
- LDL:
-
Low density lipoprotein
- DMEM:
-
Dulbecco’s modified Eagle’s medium
- FCS:
-
Fetal calf serum
- MTT:
-
3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide
- ROS:
-
Reactive oxygen species
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Acknowledgments
T. K.B. was granted by the postdoctoral fellowship of the National Foundation for Science, Higher Education and Technological Development of the Republic of Croatia. We wish to thank Dr. Silvia Roser for critically reading the manuscript. We also thank to Mrs. Doris Kappen, Mrs. Theresa Asen and Mr. Nico Gebhardt from the Chair of the Biofunctionality of Food (TUM) for their excellent technical assistance.
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Kulisic-Bilusic, T., Schnäbele, K., Schmöller, I. et al. Antioxidant activity versus cytotoxic and nuclear factor kappa B regulatory activities on HT-29 cells by natural fruit juices. Eur Food Res Technol 228, 417–424 (2009). https://doi.org/10.1007/s00217-008-0948-1
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DOI: https://doi.org/10.1007/s00217-008-0948-1