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Phenolic composition of rhubarb

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Abstract

Extracts of different parts (leaves, petioles and rhizomes) of domestic garden rhubarb (hybrids of Rheum rhabarbarum L. and Rheum rhaponticum L.) were investigated for their content of phenolic ingredients. Two stilbenes (trans-rhapontigenin, trans-desoxyrhapontigenin), five stilbene glycosides (trans-rhaponticin, cis- and trans-desoxyrhaponticin, trans-resveratrol-4′-O-β-d-glucopyranoside, trans-piceatannol-3′-O-β-d-glucopyranoside) and seven flavonoids [rutin, quercetin-3-O-glucuronide, isovitexin, 6,8-di-C-β-d-glucosylapigenin, 6-C-β-d-glucosyl-8-C-β-d-arabinosylapigenin (schaftoside), 6-C-β-d-arabinosyl-8-C-β-d-glycosylapigenin (isoschaftoside), (+)-catechin] were unequivocally established. Separation was done in two steps. Multilayer countercurrent chromatography was applied to separate different extracts of plant material. Preparative HPLC was then used to obtain pure substances. The purity and identity of isolated compounds was confirmed by different NMR experiments, HR-MS and HPLC-DAD analysis.

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Acknowledgments

We thank D. Ströhl from the Institute of Organic Chemistry, Halle (Germany), for recording NMR spectra, and J. Schmidt from the Leibnitz Institute of Plant Biochemistry, Halle (Germany), for performing accurate mass analysis.

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Authors and Affiliations

  1. Institute of Chemistry, Food Chemistry, Martin Luther University Halle-Wittenberg, Kurt-Mothes-Str. 2, 06120, Halle, Germany

    Nicole Krafczyk, Matthias Kötke, Nicole Lehnert & Marcus A. Glomb

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  1. Nicole Krafczyk
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  2. Matthias Kötke
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  3. Nicole Lehnert
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  4. Marcus A. Glomb
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Correspondence to Marcus A. Glomb.

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Krafczyk, N., Kötke, M., Lehnert, N. et al. Phenolic composition of rhubarb. Eur Food Res Technol 228, 187–196 (2008). https://doi.org/10.1007/s00217-008-0922-y

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  • DOI: https://doi.org/10.1007/s00217-008-0922-y

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