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Antioxidant activity of sulphated polysaccharide conjugates from abalone (Haliotis discus hannai Ino)


The water-soluble sulphated polysaccharide conjugates were obtained from abalone viscera (Haliotis discus hannai Ino) by alkaline protease extraction followed by ethanol precipitation. Their antioxidant activities were evaluated in vitro by hydroxyl radicals scavenging activity, reducing power and metal chelating activity. Those various antioxidant activities were compared to standard antioxidants ascorbic acid and EDTA. The experimental results indicated that the crude extract having notable hydroxyl free radicals scavenging activity and moderate reducing power and chelating potency. The crude sulphated polysaccharide conjugates was enzymatically hydrolyzed by five commercially available proteases (trypsin, vernase, neutrase, pepsin and papain), and the resultant digests were tested for their antioxidant activities. Those proteolytic hydrolysates, although improving the hydroxyl radical scavenging activity in all cases except one, had lower reducing power and 3–15 times lower chelating ability than the native extract. Product derived from pepsin hydrolysate was fractionated by gel-filtration chromatography with sephadex G-100, giving two fractions containing sulphated polysaccharide conjugates termed ACP I and ACP II. The neutral monosaccharide composition of ACP I is rhamnose, fucose, xylose, mannose, galactose and glucose in a molar ratio of 1.00:45.14:4.00:5.36:33.18:2.15, with an average molecular weight of about 271 kDa. The neutral monosaccharide composition of ACP II is rhamnose, fucose, xylose, mannose, galactose and glucose in a molar ratio of 1.00:12.51:1.33:4.98:16.08:1.46, with an average molecular weight of about 6 kDa.

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  1. Pryor WA, Ann NY (1982) Ann N Y Acad Sci 393:1–22

    Article  CAS  Google Scholar 

  2. Butterfield DA, Castenga A, Pocernich CB, Drake J, Scapagnini G, Calabrese V (2002) J Nutr Biochem 13:444–461

    Article  CAS  Google Scholar 

  3. Grice HC (1988) Food Chem Toxicol 26:717–723

    Article  CAS  Google Scholar 

  4. Becker GL (1993) Food Process 12:54–56

    Google Scholar 

  5. Finkel T, Holbrook NJ (2000) Nature 408:239–247

    Article  CAS  Google Scholar 

  6. Josephine A, Veena CK, Amudha G, Preetha SP, Varalakshmi P (2006) Mol Cell Biochem 287:101–108

    Article  CAS  Google Scholar 

  7. Park PJ, Heo SJ, Park EJ, Kim SK, Byun HG, Jeon BT, Jeon YJ (2005) J Agric Food Chem 53:6666–6672

    Article  CAS  Google Scholar 

  8. Kresge N, Vacquier VD, Stout CD (2001) Bioessays 23:95–103

    Article  CAS  Google Scholar 

  9. Zhang M, Cheung PC, Zhang L (2001) J Agric Food Chem 4:5059–5062

    Article  CAS  Google Scholar 

  10. Chen HX, Zhang M, Xie BJ (2004) J Agric Food Chem 52:3333–3336

    Article  CAS  Google Scholar 

  11. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Anal Chem 28:350–356

    Article  CAS  Google Scholar 

  12. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) J Biol Chem 193:265–275

    CAS  Google Scholar 

  13. Ghosh P, Adhikari U, Ghosal PK, Pujol CA, Carlucci MJ, Damonte EB, Ray B (2004) Phytochemistry 65:3151–3157

    Article  CAS  Google Scholar 

  14. Leung MYK, Liu C, Zhu LF, Hui YZ, Yu B, Fung KP (2004) Glycobiology 14:501–510

    Article  CAS  Google Scholar 

  15. Qi HM, Zhang QB, Zhao TT, Chen R, Zhang H, Niu XZ, Li Z (2005) Int J Biol Macromol 37:195–199

    Article  CAS  Google Scholar 

  16. Oyaizu M (1986) Jpn J Nutr 44:307–315

    CAS  Google Scholar 

  17. Dinis TC, Madeira VM, Almeida LM (1994) Arch Biochem Biophys 315:161–169

    Article  CAS  Google Scholar 

  18. Lloyd AG, Dodgson KS, Price RB, Rose FA (1961) Biochim Biophys Acta 46:108–115

    Article  CAS  Google Scholar 

  19. Wang ZJ, Luo DH (2007) Carbohydr Polym 68:54–58

    Article  CAS  Google Scholar 

  20. Tseng YH, Yang JH, Mau JL (2008) Food Chem 107:732–738

    CAS  Article  Google Scholar 

  21. Li XM, Li XL, Zhou AG (2007) Eur Polym J 43:488–497

    Article  CAS  Google Scholar 

  22. Chen Y, Xie MY, Nie SP, Li C, Wang YX (2008) Food Chem 107:231–241

    Article  CAS  Google Scholar 

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This work was financially supported by Chinese International Corporation Project (No. 2006DFA32580), Innovational Team Project of the Educational Department of Liaoning Province (No. 2006T033) and The Project of Transformation of Research Findings of the Science and Technology Department of Liaoning Province (No. 2006301008).

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Correspondence to Bei-Wei Zhu.

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Zhu, BW., Wang, LS., Zhou, DY. et al. Antioxidant activity of sulphated polysaccharide conjugates from abalone (Haliotis discus hannai Ino). Eur Food Res Technol 227, 1663 (2008).

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  • Abalone viscera (Haliotis discus hannai Ino)
  • Sulphated polysaccharide conjugates
  • Purification
  • Antioxidant activity
  • Protease