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Adhesion and aggregation properties of probiotic and pathogen strains

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Abstract

Autoaggregation has been correlated with adhesion, which is known to be a prerequisite for colonization and infection of the gastrointestinal tract by many pathogens. The coaggregation properties of probiotic strains with pathogens as well as their ability to displace pathogens are of importance for therapeutic manipulation of the aberrant intestinal microbiota. Consequently, the ability to aggregate and coaggregate are desirable properties for probiotics in health-promoting foods. Aggregation assays and bacterial adhesion to hydrocarbons (BATH test) demonstrated significant differences in cell surface properties among the tested commercial probiotic strains. Hydrophobicity increased when the cells were heat-inactivated. All probiotic strains tested showed aggregation abilities with the pathogen strains tested, but the results were strain-specific and dependent on time and incubation conditions. Our results indicate that the ability to autoaggregate, together with cell-surface hydrophobicity and coaggregation abilities with pathogen strains can be used for preliminary screening in order to identify potentially probiotic bacteria suitable for human or animal use.

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References

  1. FAO/WHO (2002) Guidelines for the evaluation of probiotics in food. Food and Agricultural Organization of the United Nations and World Health Organization. Working Group Report, 2002

  2. Collins JK, Thornton G, Sullivan GO (1998) Int Dairy J 8:487–490

    Article  Google Scholar 

  3. Ouwehand AC, Salminen S, Isolauri E (2002) Antonie van Leeuwenhoek 82:279–289

    Article  CAS  Google Scholar 

  4. Forestier C, De Champs C, Vatoux C, Joly B (2001) Res Microbiol 152:167–173

    Article  CAS  Google Scholar 

  5. Guandalini S (2006) J Clin Gastroenterol 40(3):244–248

    Article  Google Scholar 

  6. Katz JA (2006) J Clin Gastroenterol 40(3):249–255

    Article  Google Scholar 

  7. Fooks LJ, Fuller R, Gibson GR (1999) Int Dairy J 9:53–61

    Article  Google Scholar 

  8. Collado MC, González A, González R, Hernández M, Ferrús MA, Sanz Y (2005) Int J Antimicrob Agents 25:385–391

    Article  CAS  Google Scholar 

  9. Collado MC, Hernández M, Sanz Y (2005) J Food Prot 68:1034–1040

    CAS  Google Scholar 

  10. Gagnon M, Kheadr EE, Le Blay G, Fliss I (2004) Int J Food Microbiol 92:69–78

    Article  CAS  Google Scholar 

  11. Servin AL (2004) FEMS Microbiol Rev 28:405–440

    Article  CAS  Google Scholar 

  12. Freter M (1992) Factors affecting the microecology of the gut. In: Fuller R (ed) Probiotics. the scientific basis. Chapman and Hall, Glasgow, pp 111–145

    Google Scholar 

  13. Bibiloni R, Perez PF, Garrote GL, Disalvo EA, De Antoni GL (2001) Methods Enzymol 336:411–427

    Article  CAS  Google Scholar 

  14. Canzi E, Guglielmetti S, Mora D, Tamagnini I, Parini C (2005) Antonie Van Leeuwenhoek 88(3–4):207–219

    Article  CAS  Google Scholar 

  15. Boris S, Suarez JE, Barbes C (1997) J Appl Microbiol 83(4):413–420

    Article  CAS  Google Scholar 

  16. Schachtsiek M, Hammes WP, Hertel C (2004) Appl Environ Microbiol 70(12):7078–7085

    Article  CAS  Google Scholar 

  17. Rosenberg M, Gutnick D, Rosenberg M (1980) FEMS Microbiol Lett 9:29–33

    Article  CAS  Google Scholar 

  18. Kos B, Suskovic J, Vukovic S, Simpraga M, Frece J, Matosic S (2003) J Appl Microbiol 94:981–987

    Article  CAS  Google Scholar 

  19. Reniero R, Cocconcelli P, Bottazzi V, Morelli L (1992) J Gen Microbiol 138:763–768

    CAS  Google Scholar 

  20. Handley PS, Harty DW, Wyatt JE, Brown CR, Doran JP, Gibbs AC (1987) J Gen Microbiol 133(11):3207–3217

    CAS  Google Scholar 

  21. Malik A, Sakamoto M, Ono T, Kakii K (2003) J Biosci Bioeng 96:10–15

    CAS  Google Scholar 

  22. Del Re B, Sgorbati B, Miglioli M, Palenzona D (2000) Lett Appl Microbiol 31:438–442

    Article  Google Scholar 

  23. Pelletier C, Bouley C, Cayuela C, Bouttier S, Bourlioux P, Bellon-Fontaine MN (1997) Appl Environ Microbiol 63:1725–1731

    CAS  Google Scholar 

  24. Perez PF, Minnaard Y, Disalvo EA, De Antoni GL (1998) Appl Environ Microbiol 64:21–26

    CAS  Google Scholar 

  25. Collado MC, Gueimonde M, Hernandez M, Sanz Y, Salminen S (2005) J Food Prot 68(12):2672–2678

    Google Scholar 

  26. Jankovic I, Ventura M, Meylan V, Rouvet M, Elli M, Zink R (2003) J Bacteriol 185:3288–3296

    Article  CAS  Google Scholar 

  27. Vinderola CG, Medici M, Perdigón G (2004) J Appl Microbiol 96:230–243

    Article  CAS  Google Scholar 

  28. Gomez Zavaglia A, Kociubinski G, Perez P, Disalvo E, De Antoni G (2002) J Appl Microbiol 93(5):794–799

    Article  CAS  Google Scholar 

  29. Rojas M, Conway PL (1996) J Appl Bacteriol 81:474–480

    CAS  Google Scholar 

  30. Rickard AH, Gilbert P, High NJ, Kolenbrander PE, Handley PS (2003) Trends Microbiol 11:94–100

    Article  CAS  Google Scholar 

  31. Spencer RJ, Chesson A (1994) TJ Appl Bacteriol 77:215–220

    CAS  Google Scholar 

  32. Cesena C, Morelli L, Alander M, Siljander T, Tuomola E, Salminen S, Mattila-Sandholm T, Vilpponen-Salmela T, Von Wright A (2001) J Dairy Sci 84:1001–1010

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors gratefully acknowledge financial support from the Academy of Finland, Research Council for Biosciences and Environment (decision number 210309 to Åbo Akademi and 210310 to University of Turku). M.C. Collado is the recipient of Excellence Postdoctoral grant from Conselleria Empresa, Universidad y Ciencia de la Generalitat Valenciana, Spain (BPOSTDOC 06/016).

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Correspondence to Maria Carmen Collado.

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Collado, M.C., Meriluoto, J. & Salminen, S. Adhesion and aggregation properties of probiotic and pathogen strains. Eur Food Res Technol 226, 1065–1073 (2008). https://doi.org/10.1007/s00217-007-0632-x

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  • DOI: https://doi.org/10.1007/s00217-007-0632-x

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