Glucosinolate and folate content in sprouted broccoli seeds

Abstract

HPLC analysis of broccoli seeds and laboratory-grown broccoli sprouts revealed that three aliphatic glucosinolates (glucoraphanin, glucoiberin and glucoerucin) and a group of indol-glucosinolates including 4-hydroxy-glucobrassicin are preformed in the seeds. In the early stage of sprouting a reduction (approx. 20%) of the aliphatic glucosinolates was measured. During further growing, until day 8, and subsequent cold storage up to the 12th day the amounts of the aliphatic glucosinolates levelled off. While 4-hydroxy-glucobrassicin declined continuously, three minor indole-derivatives increased steadily, but remained at a comparatively low level. Besides glucosinolates, folates were quantified in broccoli sprouts by stable isotope dilution assays (SIDAs). During germination, the contents of total folates increased to 72 μg/100 g fresh mass and 546 μg/100 g dry mass on the 4th day, which was equivalent, respectively, to a 3-fold and a 24-fold increase in the seed’s content. Thereafter, total folates decreased again to 13 μg/100 g fresh mass until the 8th day of germination and remained at this low level. The folate pattern measured by SIDA revealed 5-methyltetrahydrofolate as the predominant vitamer at each stage.

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References

  1. 1.

    Fahey JW, Zhang Y, Talalay P (1997) Proc Natl Acad Sci USA 94:10367–10372

    Article  CAS  Google Scholar 

  2. 2.

    Shapiro TA, Fahey JW, Wade KL, Stephenson KK, Talalay P (2001) Cancer Epidemiol Biomark Prev 10:501–508

    CAS  Google Scholar 

  3. 3.

    Tang L, Zhang Y, Jobson HE, Li J, Stephenson KK, Wade KL, Fahey JW (2006) Mol Cancer Ther 5:935–944. doi:10.1158/1535–7163.MCT-05-0476

    Google Scholar 

  4. 4.

    Kensler TW, Chen J-G, Egner PA, Fahey JW, Jacobson LP, Stephenson KK, Ye L, Coady JL, Wang J-B, Wu Y, Sun Y, Zhang B-C, Zhu Y-R, Qian G-S, Carmella SG, Hecht S, Benning L, Gange SJ, Groopman JD, Talalay P (2005) Cancer Epidemiol Biomark Prev 14:2605–2613. doi: 10.1158/1055-9965.EPI-05-0368

  5. 5.

    Fahey JW, Zalcmann AT, Talalay P (2001) Phytochemistry 56:5–51, (2002) 59:237 (corrigendum)

  6. 6.

    Wu L, Ashraf MHN, Facci M, Wang R, Paterson PG, Ferrie A, Juurlink BHJ (2004) Proc Natl Acad Sci USA 101:7094–7099

    Article  CAS  Google Scholar 

  7. 7.

    Fahey JW, Haristoy X, Dolan PM, Kensler TW, Scholthus I, Stephenson KW, Talalay P, Lozniewski A (2002) Proc Natl Acad Sci USA 99:7610–6715

    Article  CAS  Google Scholar 

  8. 8.

    Kim DJ, Han BS, Ahn B, Hasegawa R, Shirai T, Ito N, Tsuda H (1997) Carcinogenesis 18:377–381

    Article  CAS  Google Scholar 

  9. 9.

    Fahey JW (2003) Development of novel highly chemoprotectant crucifer germplasm. US Patent 6,521,818 B1

  10. 10.

    Konings EJM, Roomans HHS, Dorant E, Goldbohm RA, Saris WHM, Van den Brandt PA (2001) Am J Clin Nutr 73:765–776

    CAS  Google Scholar 

  11. 11.

    Czeizal AF, Dudas J (1992) N Engl J Med 327:1832–1835

    Article  Google Scholar 

  12. 12.

    Snowdon DA, Tully CL, Smith CD, Riley KP, Markesbery WR (2000) Am J Clin Nutr 71:993–998

    CAS  Google Scholar 

  13. 13.

    Robinson K (2000) Heart 83:127–130

    Article  CAS  Google Scholar 

  14. 14.

    Bonaa KH, Njolstad I, Ueland PM, Schirmer H, Tverdal A, Steigen T, Wang H, Nordrehaug JE, Arnesen E, Rasmussen K (2006) New Eng J Med 354:1578–1588

    Article  CAS  Google Scholar 

  15. 15.

    Lonn E, Yusuf S, Arnold J, Sheridan P, Pogue J, Micks M, McQueen MJ, Probstfield J, Fodor G, Held C, Genest JJ (2006) New Eng J Med 354:1567–1577

    Article  CAS  Google Scholar 

  16. 16.

    Freisleben A, Schieberle P, Rychlik M (2003) Anal Biochem 315:247–255

    Article  CAS  Google Scholar 

  17. 17.

    Rychlik M (2004) J Food Comp Anal 17:475–483

    Article  CAS  Google Scholar 

  18. 18.

    Freisleben A, Schieberle P, Rychlik M (2002) J Agric Food Chem 50:4760–4768

    Article  CAS  Google Scholar 

  19. 19.

    EC (1990) Oil seeds-determination of glucosinolates, high performance liquid chromatography. Official J Eur Comm L170:28–34

    Google Scholar 

  20. 20.

    Matthaeus B, Luftmann H (2000) J Agric Food Chem 48:2234–2239. doi: 101021/jf991306w

    Google Scholar 

  21. 21.

    Rychlik M, Englert K, Kapfer S, Kirchhoff E (2006) J Food Comp Anal 20. doi:10.1016/j.jfca.2006.10.006

  22. 22.

    West LG, Meyer K, Balch BA, Rossi FJ, Schultz MR, Haas FJ (2004) J Agric Food Chem 52:916–926. doi:10.1021/jf0307189

    Google Scholar 

  23. 23.

    Rangkadilok N, Nicolas M, Bennet R, Premier R, Eagling D, Taylor P (2002) Scientia Hortic 96:11–26

    Article  CAS  Google Scholar 

  24. 24.

    Rangkadilok N, Nicolas M, Bennet R, Premier R, Eagling D, Taylor P (2002) Scientia Hortic 96:27–41

    Article  CAS  Google Scholar 

  25. 25.

    Pereira FMV, Rosa E, Fahey JW, Stephensen KK, Carvalho R, Aires A (2002) J Agric Food Chem 50:6239–6244. doi: 10.1021/jf020309x

    Google Scholar 

  26. 26.

    Plaza L, de Ancos B, Cano MP (2003) Eur Food Res Technol 216:138–144

    CAS  Google Scholar 

  27. 27.

    Liukkonen K-H, Katina K, Wilhelmsson A, Myllymaki O, Lampi A-M, Kariluoto S, Piironen V, Heinonen S-M, Nurmi T, Adlercreutz H, Peltoketo A, Pihlava J-M, Hietaniemi e, Poutanen K (2003) Proc Nutr Soc 62:117–122

    Article  CAS  Google Scholar 

  28. 28.

    Cossins EA (2000) Can J Bot 78:691–708

    Article  CAS  Google Scholar 

  29. 29.

    Roos AJ, Spronk AM, Cossins EA (1968) Can J Biochem 46:1533–1536

    CAS  Article  Google Scholar 

  30. 30.

    Dodd WA, Cossins EA (1970) Biochim Biophys Acta, General Subjects 201:461–470

    Article  CAS  Google Scholar 

  31. 31.

    Dodd WA, Cossins EA (1969) Arch Biochem Biophys 133:216–223

    Article  CAS  Google Scholar 

  32. 32.

    Rowe A (1969) In: Blakeley RL (ed) The biochemistry of folic acid and related pteridines. Wiley, New York, pp 329–344

  33. 33.

    Farhangi M, Valadon LRG (1983) J Sci Food Agric 34:1251–1256

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors express their gratitude to Christiane Winkler and Michael Meyer for growing the seeds and performing the procedures in the analysis of glucosinolates. Moreover, we are grateful to Mrs. D. Fottner for her excellent technical assistance in folate analysis. Furthermore, the valuable reviewing of the manuscript by Diana Inkster is acknowledged.

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Correspondence to Michael Rychlik.

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Rychlik, M., Adam, S.T. Glucosinolate and folate content in sprouted broccoli seeds. Eur Food Res Technol 226, 1057–1064 (2008). https://doi.org/10.1007/s00217-007-0631-y

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Keywords

  • Broccoli
  • Folates
  • glucosinolates
  • HPLC–UV
  • Sprouts
  • Stable isotope dilution assay