European Food Research and Technology

, Volume 224, Issue 2, pp 225–228 | Cite as

In vivo immunomodulatory activity of polysaccharides derived from Chlorella pyrenoidosa

  • Fangmei Yang
  • Ying Shi
  • Jianchun Sheng
  • Qiuhui HuEmail author
Original Paper


The effects of polysaccharides from Chlorella pyrenoidosa on immunomodulatory activity were investigated in this paper. Phagocytosis of peritoneal macrophages was detected through ingesting chicken red blood cells (CRBC) test to evaluate nonspecific immune response. The cellular immune response was measured using the delayed-type hypersensitivity response to sheep red blood cells (SRBC) in terms of antibody titration level. Oral administration of C. pyrenoidosa polysaccharides, 1 g and 2 g kg−1 body weight significantly enhanced phagocytic rate (23.00 and 26.25%, respectively) and phagocytic index (0.30 and 0.36, respectively) compared with saline-treated control (P<0.05). The value of ear thickness rate was greatly elevated from 10.09% in saline-treated control to 16.22 and 19.48%, respectively in group treated with 1 g and 2 g kg−1 body weight polysaccharides (P<0.05). There was a significant correlation between the dosage of C. pyrenoidosa polysaccharides and antibody titre (r=0.99, P=0.019) and the antibody titre was significantly increased by 11.80, 21.90 as well as 39.80 in 2 g kg−1 bw compared to 10.30 in the control. Dietary supplementation with C. pyrenoidosa polysaccharides did not affect the growth of the mice during the experiment. These results indicated that immunomodulatory activities in mice were enhanced by administration of polysaccharides derived from C. pyrenoidosa.


Chlorella pyrenoidosa Polysaccharide Immunomodulatory activity 



This research was supported by Jiangsu Science and Technology Department under BG 2002323 and BG 2003318.


  1. 1.
    Robledo D, Pelegrin YF (1997) Bot Mar 40(4):301–306CrossRefGoogle Scholar
  2. 2.
    Norziah MH, Ching CY (2000) Food Chem 68(1):69–76CrossRefGoogle Scholar
  3. 3.
    Aguilera-Morales M, Casas-Valdez M, Carrillo-Dominguez B, Gonzalez-Acosta B, Perez-Gil F (2005) J Food Compos Anal 18(1):79–88CrossRefGoogle Scholar
  4. 4.
    Duarte ME, Noseda DG, Noseda MD, Tulio S, Pujol CA, Damonte EB (2001) Phytomedicine 8(1):53–58CrossRefPubMedGoogle Scholar
  5. 5.
    Guven KC, Ozsoy Y, Ulutin ON (1991) Bot Mar 34(5):429–432Google Scholar
  6. 6.
    Zhang Q, Li N, Zhou G, Lu X, Xu Z, Li Z (2003) Pharmacol Res 48(2):151–155CrossRefPubMedGoogle Scholar
  7. 7.
    Trento F, Cattaneo F, Pescador R, Porta R, Ferro L (2001) Thromb Res 102(5):457–465CrossRefPubMedGoogle Scholar
  8. 8.
    Kay RA (1991) Crit Rev Food Sci Nutr 30(6):555–573PubMedCrossRefGoogle Scholar
  9. 9.
    Cherng JY, Shih MF (2005) Life Sci 76(26):3001–3013CrossRefPubMedGoogle Scholar
  10. 10.
    Kralovec JA, Power MR, Liu F, Maydanski E, Ewart HS, Watson LV, Barrow CJ, Lin TJ (2005) Int Immunopharmacol 5(4):689–698CrossRefPubMedGoogle Scholar
  11. 11.
    Miyazawa Y, Murayama T, Ooya N, Wang LF, Tung YC, Yamaguchi N (1988) J Ethnopharmacol 24(2–3):135–146CrossRefPubMedGoogle Scholar
  12. 12.
    Kralovec JA (2003) US Patent 6,551,596 B2Google Scholar
  13. 13.
    Kojima E, Lin B (2004) J Biosci Bioeng 97(5):317–321PubMedGoogle Scholar
  14. 14.
    Ma J, Zheng R, Xu L, Wang S (2002) Ecotox Environ Safe 52(1):57–61CrossRefGoogle Scholar
  15. 15.
    Liu Y, Yang HB, Lin F (2001) J Dalian Univ 22:53–56Google Scholar
  16. 16.
    Oliveira R, Marques F, Azeredo J (1999) Biotechnol Tech 13(6):391–393CrossRefGoogle Scholar
  17. 17.
    Lin PY, Feng ZM, Pan JQ, Zhang D, Xiao LY (1995) Zhongguo Yao Li Xue Bao 16(5):441–444PubMedGoogle Scholar
  18. 18.
    Noonan FP, Bucana C, Sauder DN, De Fabo EC (1984) J Immunol 132(5):2408–2416PubMedGoogle Scholar
  19. 19.
    Toews GB, Bergstresser PR, Streilein JW (1980) J Immunol 124(1):445–453PubMedGoogle Scholar
  20. 20.
    Bin-Hafeez B, Ahmad I, Haque R, Raisuddin S (2001) J Ethnopharmacol 75(1):13–18CrossRefPubMedGoogle Scholar
  21. 21.
    Cuesta G, Suarez N, Bessio MI, Ferreira F, Massaldi H (2003) J Microbiol Methods 52(1):69–73CrossRefPubMedGoogle Scholar
  22. 22.
    Ruan Z, Su J, Dai HC, Wu MC (2005) Int Immunopharmacol 5(5):811–820CrossRefPubMedGoogle Scholar
  23. 23.
    Gongora L, Manez S, Giner RM, Recio MC, Rios J-L (2000) Life Sci 66(14):PL183–PL188CrossRefPubMedGoogle Scholar
  24. 24.
    Han SB, Kim YH, Lee CW, Park SM, Lee HY, Ahn KS, Kim IH, Kim HM (1998) Immunopharmacology 40(1):39–48CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Fangmei Yang
    • 1
  • Ying Shi
    • 1
  • Jianchun Sheng
    • 1
  • Qiuhui Hu
    • 1
    Email author
  1. 1.Laboratory of Green Food, College of Food Science and TechnologyNanjing Agricultural UniversityNanjingP.R. China

Personalised recommendations