The purpose of this work was to study the influence of the industrial process steps on free fatty acids, peroxide value (PV), p-anisidine value (PAV), trans fatty acids, tocopherols and sterols (free, esterified and total) in high oleic safflower oil. Degumming, bleaching and deodorization steps removed 91.4% of free fatty acids, 96.31% of oxidation primary products (PV), and 54.57% of oxidation secondary products (PAV), from crude high oleic safflower oil. Degumming neither affected the content of sterified sterols nor its proportion with respect to the crude oil. A significant increment (p<0.05) in the content of free sterols was observed during degumming and bleaching due to the acid-catalyzed hydrolysis of steryl esters. A significant reduction (p<0.05) in the content of total sterols during bleaching was observed, which is attributed to a reduction in the sterified sterol fraction. During deodorization, free sterols were distilled from oil, with a gradual reduction in the total sterol content as a function of the deodorization temperature. α- and γ-tocopherols represented 93.3% of the total tocopherols in high oleic safflower crude oil. The refining process removed 28.5% of the tocopherols. Deodorization was the main step which increased the level of trans fatty acids as an effect of temperature and heating time.
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Lechner M, Reiter B, Lorbeer E (1999) J Chromatogr A 857:231–238
Ferrari R, Schulte E, Esteves W, Brühl L, Mukherjee K (1996) J Am Oil Chem Soc 73:587–591
Bockisch M (1998) Fats and oils handbook. AOCS Press, Champaign, IL
Decap P, Braipson-Danthine S, Vanbrabant B, De Greyt W, Deroanne C (2004) J Am Oil Chem Soc 81:611–617
Zschau W (2000) Bleaching. In: OBrien RD, Farr W Wan P (eds) Introduction to fats and oils technology, 2nd edn. AOCS Press, Champaign, IL, pp 215–235
Zehnder C (1995) Deodorization. In: Erickson D (ed) Practical handbook of soybeans processing and utilization. AOCS Press, Champaign, IL, pp 240–244
Kellens M and De Greyt W (2000) Deodorization. In: O'Brien R, Farr W, Wan P (eds) Introduction to fats and oils technology. AOCS Press, Champaign, IL, pp 235–268
Kamal-Eldin A, Appelqvist L, (1996) Lipids 31:671–701
Verleyen T, Forcades M, Verhe R, Dewettinck K, Huyghebaert A, De Greyt W (2002) J Am Oil Chem Soc 79:117–122
Grob K, Biederman M, Artho A, Schmid J (1994) Riv It Sost Grasse 71:533–538
Firestone D (1998) American Oil Chemists' Society (AOCS) official methods and recommended practices of American Oil Chemists' Society. Method Ca 8-53. Peroxide value; method Ca 5a-40. Free fatty acids; method Cd 18-90. p-anisidine value; method Ce 2-66. Preparation of methyl esters of fatty acids, 5th edn. AOCS Press, Champaign, IL
Sanchez-Machado D, Lopez-Hernandez J, Paseiro-Losada P, Lopez-Cervantes J (2004) Biomed Chromatogr 18:83–190
Gemeinschaftarbeitung DGF (1990) Fat Sci Technol 92:189–192
Paquot C, Haufnne A (1992) International Union of Pure and Applied Chemistry (IUPAC) standard methods for the analysis of oils, fats and derivates: Method 2.432. Determination of tocopherols by HPLC, 7th edn. Blackwell, Oxford, pp 877–892
Medina-Juarez L, Gámez-Meza N, Ortega-García J, Noriega-Rodriguez J, Angulo G, (2000) J Am Oil Chem Soc 77:721–724
Smith J (1996) Safflower. AOCS Press, Champaign, IL.
Rossi M, Gianazza M, Alamprese C, Stanga F (2003) Food Chem 82:291–296
Serra B, Soliva T, Ventura C, (2001) J Am Oil Chem Soc 78:305–310
White P (1994) Conjugated diene, anisidine value, and carbonyl value analyses. In: Warner K, Eskin N (eds) Methods to asses quality and stability and stability of oils and fat-containing foods. AOCS Press, Champaign, IL, pp 159–189
Grob K, Artho A, Mariani C (1992) Fat Sci and Technol 94:394–399
Duchateau G, van Oosten H, Vasconcellos M (1996) J Am Oil Chem Soc 73:275–282
Wolff R (1992) J Am Oil Chem Soc 69:106–110
Wolff R (1994). J Am Oil Chem Soc 71:1129–1134
Wolff R (1994) J Am Oil Chem Soc 71:907–909
We express our sincere appreciation to the Consejo Nacional de Ciencia y Tecnología (CONACYT), and the Refining Oil Industry of Mexico for the support and encouragement for this work.
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Ortega-García, J., Gámez-Meza, N., Noriega-Rodriguez, J.A. et al. Refining of high oleic safflower oil: Effect on the sterols and tocopherols content. Eur Food Res Technol 223, 775–779 (2006). https://doi.org/10.1007/s00217-006-0267-3
- High oleic safflower
- Free fatty acids
- Peroxide value
- p-anisidine value
- trans Fatty acids
- Tocopherols and sterols