The biological control capability of strain 34-9 of Kloeckera apiculata against Penicillium italium (Wehmer), postharvest rot of citrus fruits and Botrytis cinerea, postharvest rot of grape fruits was studied in vitro and in vivo. Strain 34-9 of K. apiculata at 3×108 CFU (colony-forming unit)/ml of washed cells provided complete control of 3×105 spores/ml of P. italium and B. cinerea during storage at 25 °C for 6 d. Antagonist population increased 40, 195 times in citrus fruit wound site and grape fruit wound site at 25 °C for 3 d, respectively, then the population stabilized for the remaining storage period. Cell-free culture filtrate, supernatant fluid and sterilized solution of strain 34-9 of K. apiculata had no antagonist against P. italium of citrus and B. cinerea of grape. These results showed that competition for nutrient, not antibiotic production, played a major role in the biological control capability of strain 34-9 of K. apiculata against P. italium of citrus and B. cinerea of grape.
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This research was financially supported by a grant from the National Natural Science Foundation of China to B.D. (NSFC No. 30371018)
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Long, CA., Wu, Z. & Deng, BX. Biological control of Penicillium italicum of Citrus and Botrytis cinerea of Grape by Strain 34–9 of Kloeckera apiculata. Eur Food Res Technol 221, 197–201 (2005). https://doi.org/10.1007/s00217-005-1199-z