Skip to main content
SpringerLink
Log in

Antioxidant activity of enzymatic extracts from a brown seaweed Ecklonia cava by electron spin resonance spectrometry and comet assay

  • Original Paper
  • Published:
European Food Research and Technology Aims and scope Submit manuscript

Abstract

The potential antioxidant activity of enzymatic extracts from Ecklonia cava was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl and alkyl radical scavenging using a electron spin resonance (ESR) spectrophotometer compared with ESR signal intensity and inhibitory effect of DNA damage on comet assay. E. cava was enzymatically hydrolyzed with five food industrial carbohydrases (AMG, Celluclast, Termamyl, Ultraflo and Viscozyme) from Novozyme Nordisk. All the tested extracts exhibited strong radical scavenging activities and the values were dose-dependent. The DPPH and hydroxyl radical scavenging activities of the enzymatic extracts were compatible with the vitamin C, which the alkyl radical scavenging activity was even higher than that of vitamin C. Also in the present study, the enzymatic extracts showed strong inhibitory effect against DNA damage in a dose dependent manner. These results indicated that the E. cava might be valuable natural antioxidative source.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

Reference

  1. Brand MD (2000) Exp Gerontol 35:811–820

    Article  CAS  PubMed  Google Scholar 

  2. El-Habit OHM, Saada HN, Azab KS (2000) Mutat Res 466:179–186

    CAS  PubMed  Google Scholar 

  3. Jun WJ, Han BK, Yu KW, Kim MS, Chang IS, Kim HY, Cho HY (2001) Food Chem 75:439–444

    Article  CAS  Google Scholar 

  4. Hettiarachchy NS, Glenn KC, Gnanasambandam R, Johnson MG (1996) J Food Sci 61:516–519

    CAS  Google Scholar 

  5. Frlich I, Riederer P (1995) Drug Res 45:443–449

    Google Scholar 

  6. Kinsella JE, Frackel E, German B, Kanner J (1993) Food Technol 47:85–89

    CAS  Google Scholar 

  7. Lai LS, Chou ST, Chao WW (2001) J Agr Food Chem 49:963–968

    Article  CAS  Google Scholar 

  8. Dykens JA, Shick JM, Benoit C, Buettner GR, Winston GW (1992) J Exp Biol 168:219–241

    CAS  Google Scholar 

  9. Sukenik A, Zmora O, Carmeli Y (1993) Aquaculture 177:313–326

    Article  Google Scholar 

  10. Matsukawa R, Dubinsky Z, Kishimoto E, Masak K, Masuda Y, Takeuchi T, Chihara M, Yamamoto Y, Niki E, Karube I (1997) J Appl Phycol 9:29–35

    Google Scholar 

  11. Yan XJ, Li XC, Zhou CX, Fan X (1996) J Appl Phycol 8:201–203

    Google Scholar 

  12. Yan XJ, Chuda Y, Suzuki M, Nagata T (1999) Biosci Biotechnol Biochem 63:605–607

    Article  CAS  PubMed  Google Scholar 

  13. Yoshie Y, Wang W, Hsieh YP, Suzuki T (2002) J Tokyo University of Fisheries 88:21–24

    Google Scholar 

  14. Takashi N, Akihiro F, Terukazu N, Michio F, Eisuke K (1999) Thromb Res 96:37–49

    Article  PubMed  Google Scholar 

  15. Takashi N, Tomoko Y, Mariko H, Terukazu N, Haruo S (2000) Thromb Res 99:623–634

    Article  PubMed  Google Scholar 

  16. Satoru K, Noboru T, Hiroo N, Shinji S, Hiroshi S (2003) Biochem Phamacol 65:173–179

    Article  Google Scholar 

  17. Heo SJ, Lee KW, Song CB, Jeon YJ (2003a) Algae 18(1):71–81

    Google Scholar 

  18. Heo SJ, Jeon YJ, Lee JH, Kim HT, Lee KW (2003b) Algae 18(4):341–347

    Google Scholar 

  19. Nanjo F, Goto K, Seto R, Suzuki M, Sakai M, Hara Y (1996) Free Radic Bio Med 21:895–902

    Article  CAS  Google Scholar 

  20. Rosen GM, Rauckman EJ (1984) Spin trapping of superoxide and hydroxyl radicals. In: Packer L (ed) Methods in enzymology. Academic Press, Orlando, FL

    Google Scholar 

  21. Hiramoto K, Johkoh H, Sako KI, Kikugawa K (1993) Free Radic Res Commun 19:323–332

    CAS  PubMed  Google Scholar 

  22. Singh NP, Graham MM, Singh V, Khan A (1995) Int Radiat Biol 68:563–569

    CAS  Google Scholar 

  23. Ramos EAP, Xiong YL (2002) J Food Sci 67:2952–2956

    Google Scholar 

  24. Rupérez P, Ahrazem O, Leal JA (2002) J Agr Food Chem 50:840–845

    Article  Google Scholar 

  25. Park PJ, Shahidi F, Jeon YJ (2004) J Food Lipids 11:15–27

    Google Scholar 

  26. Lu Y, Foo YL (2000) Food Chem 68:81–85

    Article  CAS  Google Scholar 

  27. Kim KY, Guo Q, Packer L (2002) J Toxicol 172:149–156

    Article  CAS  Google Scholar 

  28. Federica P, Stefania B, Lara M, Michele M, Fabrizia S (2004) J Pharm Biomed 35:289–301

    Article  Google Scholar 

  29. Ruberto G, Baratta MT, Biondi DM, Amico V (2001) J Appl Physiol 13:403–407

    Google Scholar 

  30. Zhang D, Yasuda T, Yu Y, Zheng P, Kawabata T, Ma Y, Okada S (1996) Free Radic Bio Med 20:145–150

    Article  CAS  Google Scholar 

  31. Ahn CB, Jeon YJ, Kang DS, Shin TS, Jung BM (2004) Food Res Int 37:253–258

    Article  CAS  Google Scholar 

  32. Kassie F, Pqrzefall W, Knasmuller S (2000) Mutat Res 463:13–31

    CAS  PubMed  Google Scholar 

  33. Donelly ET (1999) Mutagenesis 14:505–512

    Article  PubMed  Google Scholar 

  34. Heo SJ, Park EJ, Lee KW, Jeon YJ (2004) Bioresour Technol (in press)

  35. Betii C, Davini T, Giannessi L, Loprieno N, Barale R (1995) Mutat Res 307:201–207

    Google Scholar 

  36. Hartmann A, Herkommer K, Gluck M, Speit G (1995) Environ Mol Mutagen 25:180–187

    CAS  PubMed  Google Scholar 

  37. Binkova B, Lewtas J, Miskova I, Rossner P, Cerna M, Mrackova G, Peterkova K, Mumford J, Meyer S, Sram R (1996) Environ Health Persp 104:591–597

    CAS  Google Scholar 

  38. Zhang H, Spitz MR, Tomlinson GE, Schabath MB, Minna JD, Wu X (2002) Cancer Detect Prev 26:411–419

    Article  CAS  PubMed  Google Scholar 

  39. Senthilmohan ST, Zhang J, Stanley RA (2003) Nutr Res 23:1199–1210

    Article  CAS  Google Scholar 

  40. Bagchi D, Bagchi M, Stohs SJ, Das DK, Ray SD, Kuszynski CA, Joshi SS, Pruess HG (2000) J Toxicol 148:187–197

    Article  CAS  Google Scholar 

  41. Athukorala A, Lee KW, Song CB, Ahn CB, Shin TS, Cha YJ, Shahidi F, Jeon YJ (2003) J Food Lipids 10:251–265

    CAS  Google Scholar 

  42. Siriwardhana N, Lee KW, Kim SH, Ha JW, Jeon YJ (2003) Food Sci Tech Int 9:339–346

    Article  Google Scholar 

Download references

Acknowledgement

This work was supported by a grant (p-2004-03) from Marine Bioprocess Research Center of the Marine Bio 21 Center funded by the Ministry of Maritime Affairs & Fisheries, Republic of Korea and Taerim Co., Ltd.

Author information

Authors and Affiliations

  1. Faculty of Applied Marine Science, Cheju National University, Jeju, 690-756, South Korea

    Soo-Jin Heo & You-Jin Jeon

  2. Department of Biotechnology, Konkuk University, Chungju, 380-701, South Korea

    Pyo-Jam Park

  3. Department of Chemistry, Pukyong National University, Pusan, 608-737, South Korea

    Se-Kwon Kim

  4. Division of Life Sciences, Kyungnam University, Masan, 631-701, South Korea

    Eun-Ju Park

Authors
  1. Soo-Jin Heo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Pyo-Jam Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Eun-Ju Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Se-Kwon Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. You-Jin Jeon
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to You-Jin Jeon.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Heo, SJ., Park, PJ., Park, EJ. et al. Antioxidant activity of enzymatic extracts from a brown seaweed Ecklonia cava by electron spin resonance spectrometry and comet assay. Eur Food Res Technol 221, 41–47 (2005). https://doi.org/10.1007/s00217-005-1187-3

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00217-005-1187-3

Keywords

Search

Navigation