Abstract
The interactions between soy and wheat proteins after dough mixing and bread baking were studied. Protein extractions from gluten and breadcrumbs elaborated with mixtures of wheat flour and enzyme-active full-fat, heat-treated full-fat, and enzyme-active defatted soy flours, and commercial isolated soy proteins were analyzed by electrophoresis. Different buffer systems with specific chemical action on proteins were used to investigate the types of forces between soy and wheat proteins in gluten and breadcrumbs. The presence of protein bands of similar molecular weight to soy proteins in gluten fractions after intensive dough washing indicated association between these proteins. Nonreduced wheat–soy gluten proteins had higher amount of sodium dodecyl sulfate soluble protein aggregates than wheat gluten. Soy and wheat proteins interacted by means of noncovalent and covalent (disulfide) bonds and the extent of the interactions depended on the soy protein state.
Similar content being viewed by others
References
Friedman M, Brandon DL (2001) J Agric Food Chem 49:1069–1086
Shewry PR, Tatham AS (1994) In Martino Cimino (eds) Wheat kernel proteins: molecular and functional aspects. University of Tucsia, Viterbo, Italy, pp 19–26
Fleming S, Sosulski F (1978) Cereal Chem 55:373–382
Dhingra S, Jood S (2002) Food Chem 77:479–488
Doxastakis G, Zafiriadis I, Irakli M, Marlani H, Tananaki C (2002) Food Chem 77:219–227
Hyder M, Hoseney RC, Finney K, Shogren M (1974) Cereal Chem 5:666–674
Lampart-Szczapa E, Jankiewicz M (1983) Food Chem 10:97–109
Ryan K, Homco-Ryan C, Jenson J, Robbins K, Prestat C, Brewer M (2002) Cereal Chem 79:434–438
Ryan KJ, Brewer MS (2005) Food Chem 89:109–124
Ribotta PD, Arnulphi SA, León AE, Añón MC (2004) J Sci Food Agric 84:1969–1974
Ng P, Bushuk W (1987) Cereal Chem 64:324–327
Laemmli V (1970) Nature 227:680–685
Khan K, Huckle L, Freeman T (1994) Cereal Chem 71:242–247
Garfin D (1994) In Deutscher MP (ed) Guide to Protein Purification (Methods in Enzymology Series) vol 182. Academic Press Inc., Orlando, Florida, pp 425–441
Añón MC, Sorgentini D, Wagner J (2001) J Agric Food Chem 49:4852–4858
Acknowledgements
The authors would like to thank the Laboratorio de Idiomas (FCA-UNC) for providing useful suggestions to improve the English in this paper and the Agencia Córdoba Ciencia SE and the Agencia Nacional de Promoción Científica y Tecnológica for financial support.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Ribotta, P.D., León, A.E., Pérez, G.T. et al. Electrophoresis studies for determining wheat–soy protein interactions in dough and bread. Eur Food Res Technol 221, 48–53 (2005). https://doi.org/10.1007/s00217-005-1135-2
Received:
Revised:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00217-005-1135-2