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Effect of dry heated inulin on selected intestinal bacteria

Abstract

Degradation of a sample of high-molecular (degree of polymerisation, DP, between 13 and 30) and low-molecular (DP below 12) inulin from Jerusalem artichoke during dry heating for 30 min at 165 and 195 °C was analysed using high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and thin layer chromatography. Dry heating at 195 °C induced complete degradation of the fructan chains and the concomitant formation of low-molecular degradation products, most likely di-d-fructose dianhydrides. In vitro fermentation studies using mixed faecal samples of eight human volunteers for 24 h at 37 °C showed significant stimulation of the growth of bifidobacteria and Enterobacteriaceae and a significant decrease of possibly pathogenic bacteria of the Clostridium histolyticum and C. lituseburense group by inulin samples heated at 195 °C compared to unheated samples and samples heated at 165 °C. This preliminary data may point to the hypothesis that heat-treated inulin or its degradation products may cause improvements of the gut microflora superior to native inulin.

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Correspondence to T. Henle.

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Böhm, A., Kleessen, B. & Henle, T. Effect of dry heated inulin on selected intestinal bacteria. Eur Food Res Technol 222, 737–740 (2006). https://doi.org/10.1007/s00217-005-0184-x

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  • DOI: https://doi.org/10.1007/s00217-005-0184-x

Keywords

  • Inulin
  • Thermal treatment
  • Bifidobacteria
  • Di-d-fructose dianhydride