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Chemical composition and antioxidant activity of tronchuda cabbage internal leaves

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Abstract

A phytochemical study was undertaken on the internal leaves of tronchuda cabbage (Brassica oleracea L. var. costata DC). Seventeen phenolic compounds were characterized and quantified by reversed-phase HPLC-DAD-ESI-MSn and HPLC/DAD, respectively: quercetin 3-O-sophoroside-7-O-glucoside, 3-p-coumaroylquinic acid, kaempferol 3-O-sophoroside-7-O-glucoside, kaempferol 3-O-(caffeoyl)-sophoroside-7-O-glucoside, sinapoyl gluc-oside acid, kaempferol 3-O-(sinapoyl)-sophoroside-7-O-glucoside, kaempferol 3-O-(feruloyl)-sophoroside-7-O-glucoside, kaempferol 3-O-(p-coumaroyl)-sophoroside-7-O-glucoside, 4-p-coumaroylquinic acid, sinapic acid, kaempferol 3-O-sophoroside, 3 isomeric forms of 1,2-disinapoylgentiobiose, 1-sinapoyl-2-feruloylgentiobiose, 1,2,2-trisinapoylgentiobiose and 1,2′-disinapoyl-2-ferul-oylgentiobiose. Seven organic acids (aconitic, citric, ascorbic, malic, quinic, shikimic and fumaric acids) were also identified and quantified. The hot water extract of tronchuda cabbage internal leaves was investigated for its capacity to act as a scavenger of DPPH• radical and reactive oxygen species (superoxide radical, hydroxyl radical and hypochlorous acid), exhibiting antioxidant capacity in a concentration dependent manner against all radicals.

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Acknowledgements

The authors are grateful to Fundação para a Ciência e Tecnologia (POCI/AGR/57399/2004) for financial support of this work. Vendula Vrchovská is grateful to European Union Erasmus/Socrates II Programme for a grant (MSM 002 162 0822).

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Authors and Affiliations

  1. Research Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS (CSIC), Campus University Espinardo, P.O. Box 164, 30100, Murcia, Spain

    Federico Ferreres

  2. REQUIMTE/ Serviço de Farmacognosia, Faculdade de Farmácia, Universidade do Porto, R. Aníbal Cunha, 164, 4050-047, Porto, Portugal

    Carla Sousa, Patrícia Valentão, Rosa M. Seabra & Paula B. Andrade

  3. Department of Pharmacognosy, Faculty of Pharmacy, Charles University, Heyrovského 1203, 500 05, Hradec Králové, Czech Republic

    Vendula Vrchovská

  4. CIMO/ESAB, Quinta de Sta Apolónia, Apartado 1172, 5301-855, Bragança, Portugal

    José A. Pereira

Authors
  1. Federico Ferreres
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  2. Carla Sousa
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  3. Vendula Vrchovská
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  4. Patrícia Valentão
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  5. José A. Pereira
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  6. Rosa M. Seabra
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  7. Paula B. Andrade
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Corresponding author

Correspondence to Paula B. Andrade.

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Ferreres, F., Sousa, C., Vrchovská, V. et al. Chemical composition and antioxidant activity of tronchuda cabbage internal leaves. Eur Food Res Technol 222, 88–98 (2006). https://doi.org/10.1007/s00217-005-0104-0

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  • DOI: https://doi.org/10.1007/s00217-005-0104-0

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