Abstract
Samples artificially infected by Fusarium culmorum were analyzed to assess the effects of Fusarium infection on the protein quality of winter wheat (Triticum aestivum L.). The Fusarium infection did not noticeably influence either the crude protein content or the water absorption ability of the wheat flour. The protease activity found in the wheat flour was inversely correlated to the sedimentation value. In contrast, it was positively correlated to both the free amino acid content and the degree of infection as expressed in Fusarium protein equivalents (FPE) quantified by an enzyme-linked immunosorbent assay. A distinct reduction in the content of both total glutenin and high molecular weight glutenin subunits was detected in the seriously infected samples (FPE>20 µg g−1). The infection with Fusarium impaired the dough quality and led to a deformed loaf shape. The fungal protease was active over both a wide range of temperatures (from 10 to 100 °C) and a wide range of pH values (from 4.5 to 8.5). The maximum protease activity was displayed at 50 °C in the pH range 6.0–8.0. This property indicates that the protease produced by F. culmorum may impair storage proteins throughout all the processing procedures associated with wheat flour, thereby causing weak dough properties and, consequently, unsatisfactory bread quality.
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References
Meyer D, Weipert D, Mielke H (1986) Getreide Mehl Brot 40:35–39
McMullen MP, Schartz B, Stover R, Gregoire T (1997) Cereal Research Commun, vol 25. Mesterhazy A (ed) Cereal Research Institute, Szeged, Hungary, pp 779–780
Parry DW, Jenkinson P, McLeod L (1995) Plant Pathol 44:207–238
Rotter BA, Prelusky DB, Pestka JJ (1996) J Toxicol Environ Health 48:1–34
Ehling G, Cockburn A, Snowdon P, Buchhaus H (1997) Cereal Res Commun 25:433–447
Eriksen GS, Alexander J (1998) Nordic Council of Ministers, Copenhagen
Lemmens M, Haim K, Lew H, Ruckenbauer P (2004) J Phytopathol 152:1–8
Bechtel DB, Kaleikau LA, Gaines RL, Seitz LM (1985) Cereal Chem 62:191–197
Boyacioglu D, Hettiarachchy NS (1995) J Cereal Sci 21:57–62
Dexter JE, Clear RM, Preston KR (1996) Cereal Chem 73:695–701
Dexter JE, Marchylo BA, Clear RM, Clarke JM (1997) Cereal Chem 74:519–525
Pawelzik E, Permady H, Weinert J, Wolf GA (1998) Getreide Mehl und Brot 52:264–266
Wieser H (2000) Cereal Chem 77:48–52
Al-Kubrusli R (2000) PhD thesis, Göttingen, Germany
Sweeney RA, Rexroad PR (1987) J AOAC 70:1028–1030
Wang Z (2000) ISBN 7-5019-2766-9, China Light Industry Publishing House, Beijing, 369–371
Standard Methods of the International Association for Cereal Science and Technology (ICC) (1999) Vienna
Wirth SJ, Wolf GA (1992) Soil Biochem 24:511–519
Wieser H, Antes S, Seilmeier W (1998) Cereal Chem 75:644–650
Wieser H, Hsam SLK, Zeller F (2003) Cereal Chem 80:247–251
Anonymous (1993) Millipore Waters Chromatograph. Manual Number WAT052874TP
Herbert P, Barros P, Ratola N, Alves A (2000) J Food Sci 65:1130–1133
Cohen SA, Michaud DP (1993) Anal Biochem 211:279–287
Kieffer R, Belitz HD, Zweier M, Ipfelkofer R, Fischbeck G (1993) Z Lebensm Unters Forsch 197:134–136
Wang J, Lege, Wolf GA, Pawelzik E (2002) VDLUFA-Kongressband, 2002 Leipzig, VDLUFA-Schriftreihe 158:680–683
Matthäus K, Dänicke S, Vahjen W, Simon O, Wang J, Valenta H, Meyer K, Strumpf A, Ziesenib H, Flachowsky G (2004) Arch Anim Nutr 58:19–35
Mielke H, Meyer D (1990) Nachr bl Dtsch Pflanzenschutzd 42:161–170
Schwarz PB, Schwarz JG, Zhou A, Prom LK, Steffenson BJ (2001) Monatsschr Brauwiss 3:55–63
Hermann W, Aufhammer W, Kübler E, Kaul HP (1999) Pflanzenbauwissenschaften 3:82–87
Hamilton RMG, Trenholm HL (1984) Anim Feed Sci Tech 11:293–300
Nightingale M, Marchylo BA, Clear RM, Dexter JE, Preston KR (1999) Cereal Chem 76:150–158
Simmonds PM (1968) Technical Bulletin No. 1. Research Branch, Canada Agriculture Saakatoon
Huebner FR, Kaczkowski J, Bietz JA (1990) Cereal Chem 67:464–470
Belitz HD, Grosch W, Schieberle P (2001) Springer Berlin, Heideberg, New York pp 258–260, 681
Acknowledgements
J.W. was supported under the Law of Lower Saxony for the Promotion of Graduate Students in the Arts and Sciences (Graduiertenförderungsgesetz), by the International PhD Program for Agricultural Sciences in Göttingen (IPAG) and by the German Academic Exchange Service (DAAD).
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Wang, J., Wieser, H., Pawelzik, E. et al. Impact of the fungal protease produced by Fusarium culmorum on the protein quality and breadmaking properties of winter wheat. Eur Food Res Technol 220, 552–559 (2005). https://doi.org/10.1007/s00217-004-1112-1
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DOI: https://doi.org/10.1007/s00217-004-1112-1