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Anticoagulant activity of heterochitosans and their oligosaccharide sulfates

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Abstract

Three kinds of partially deacetylated heterochitosans, 90% deacetylated chitosan, 75% deacetylated chitosan and 50% deacetylated chitosan, were prepared from crab chitin by N-deacetylation with 40% sodium hydroxide solution for different durations. Nine kinds of heterochitooligosaccharides (hetero-COSs) with relatively high molecular weights (5,000–10,000 Da; 90-HMWCOSs, 75-HMWCOSs, and 50-HMWCOSs), medium molecular weights (1,000–5,000 Da; 90-MMWCOSs, 75-MMWCOSs, and 50-MMWCOSs), and low molecular weights (below 1,000 Da; 90-LMWCOSs, 75-LMWCOSs, and 50-LMWCOSs) were prepared using an ultrafiltration membrane reactor system, respectively. In addition, their sulfated derivatives were prepared by a method using a trimethylamine-sulfur trioxide, and the anticoagulant properties of the heterochitosans and their COS sulfates with different chain lengths and degrees of deacetylation were investigated. Clotting times in thrombin-time assay were prolonged in the presence of various concentrations of the heterochitosans and their COS sulfates using normal human plasma. The 90% deacetylated chitosan sulfate exhibited the highest anticoagulant activity among all the heterochitosans and their COS sulfates.

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Acknowledgement

This work was supported by the Brain Korea21 project in 2003.

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Authors and Affiliations

  1. Department of Chemistry, Pukyong National University, 608–737, Busan, South Korea

    Pyo-Jam Park, Jae-Young Je, Won-Kyo Jung & Se-Kwon Kim

  2. Department of Food Science and Nutrition, Yosu National University, 550–749, Yosu, South Korea

    Chang-Bum Ahn

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  1. Pyo-Jam Park
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  2. Jae-Young Je
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  3. Won-Kyo Jung
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  5. Se-Kwon Kim
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Correspondence to Se-Kwon Kim.

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Park, PJ., Je, JY., Jung, WK. et al. Anticoagulant activity of heterochitosans and their oligosaccharide sulfates. Eur Food Res Technol 219, 529–533 (2004). https://doi.org/10.1007/s00217-004-0977-3

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  • DOI: https://doi.org/10.1007/s00217-004-0977-3

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