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Photobacterium phosphoreum isolated as a luminescent colony from spoiled fish, cultured in model system under controlled atmospheres

Abstract.

A luminescent colony isolated from spoiled hake, tentatively identified as Photobacterium phosphoreum, was cultured in a sterile fish juice at 1±1 °C. Growth, trimethylamine, off-odour and biogenic amines production under four gas mixtures (60% CO2/15% O2/25% N2, 60% CO2/40% O2, 40% CO2/60% O2, 40% CO2/40% O2/20% N2) and under air were tested. Aerobically incubated bacteria showed the highest counts and trimethylamine production after 43 days of storage (>108 cfu/ml and 28 mg TMA-N/100 ml, respectively). Mixtures containing CO2 and O2 seemed to exert an inhibitory effect both on growth and trimethylamine production by this bacteria, specially for mixtures containing 60% CO2. Off-odour production was detected when the bacteria was incubated in air from day 15. Cadaverine was the major amine produced by P. phosphoreum followed by histamine. Putrescine and spermidine production generally increased during the aerobic incubation. However, the level of histamine and tyramine was higher under the gas mixture containing 60% CO2/15% O2/25% N2 than in air, whereas the agmatine production seemed to be enhanced under any gas mixture assayed.

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López-Caballero, M., Álvarez Torres, M., Sánchez-Fernández, J. et al. Photobacterium phosphoreum isolated as a luminescent colony from spoiled fish, cultured in model system under controlled atmospheres. Eur Food Res Technol 215, 390–395 (2002). https://doi.org/10.1007/s00217-002-0575-1

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  • DOI: https://doi.org/10.1007/s00217-002-0575-1

  • Photobacterium phosphoreum Fish spoilage Controlled atmospheres Biogenic amines