Abstract
A new ion chromatographic (IC) technique has been developed for the determination of inorganic cations in biological fluids with direct sample injection. This involved the use of a mixed zwitterionic-micelle/electrolyte solution as an eluent. The proteins in the sample became bound to the zwitterionic micelles in the eluent and were thus eliminated from the column. The cations were separated by cation exchange. This method is ideal for the on-line, simultaneous determination of common inorganic cations (Na+, NH4 +, K+, Mg2+, and Ca2+) in urine and serum samples. Such an application was demonstrated experimentally. Non-suppressed conductivity was used for analyte detection. The detection limits obtained using this IC system were 2.94, 5.22, 34.9, 32.6, and 56.7 μg/L for Na+, NH4 +, K+, Mg2+, and Ca2+, respectively.
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Received: 10 November 2000 / Revised: 5 February 2001 / Accepted: 8 February 2001
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Hu, W., Matsukami, H., Iles, A. et al. Use of zwitterionic micelles in the eluent II: a new approach to ion chromatographic analysis of inorganic cations in biological fluids with direct sample injection. Fresenius J Anal Chem 370, 426–428 (2001). https://doi.org/10.1007/s002160100781
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DOI: https://doi.org/10.1007/s002160100781