Abstract
The relationships between electromigration dispersion (EMD) and on-line isotachophoresis-capillary zone electrophoresis (ITP-CZE) are described for several basic model proteins and interleukin-6 (rhIL-6). During CZE separation of the highly concentrated analyte zones which were generated during the initial ITP step EMD evolves from intrinsic differences in conductivity between the focused ITP zones and the leading electrolyte. Nearly triangular peaks with a sharp front and diffuse rear side were observed. An electromigration dispersion factor (FEMD) was introduced to measure peak asymmetry. EMD of individual peaks was shown to increase with the absolute amount of the respective analyte injected and with analyte mobility. Good linearity was observed when FEMD was plotted against protein mobility (r > 0.95). The slope of the graphs describing this relationship increased with the amount of analyte injected. The influence of EMD on the separation efficiency of neighboring peaks appeared to be less pronounced than expected. Consecutive release from the ITP-stack during transition from ITP to CZE might be an explanation for this observation.
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Received: 18 January 1999 / Revised: 14 April 1999 / Accepted: 3 May 1999
Rights and permissions
About this article
Cite this article
Gysler, J., Jaehde, U. & Schunack, W. Electromigration peak dispersion of isotachophoretic protein zones during capillary zone electrophoresis. Fresenius J Anal Chem 365, 398–403 (1999). https://doi.org/10.1007/s002160051630
Issue Date:
DOI: https://doi.org/10.1007/s002160051630