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Fresenius' Journal of Analytical Chemistry

, Volume 366, Issue 6–7, pp 669–676 | Cite as

Nano-electrospray ionization mass spectrometry: addressing analytical problems beyond routine

  • M. Karas
  • U. Bahr
  • T. Dülcks
Review

Abstract

The advent of nano-electrospray ionization (nano-ESI) has considerably extended the usability of ESI in the analytical mass spectrometric laboratory. One of the remarkable features of nano-ESI is its extremely low sample consumption. Only a few microliters of analyte solution (10–5–10–8 M) are sufficient for molecular weight determination and structural investigations by MS/MS. But nano-ESI is more than just a minimized-flow ESI; the low solvent flow rate also affects the mechanism of ion formation. As a consequence, the area of ESI-MS applications is significantly enhanced. Oligosaccharides, glycosides as well as glycoproteins can be analyzed more easily than with normal ion spray. The same holds for the analysis of non-covalent complexes sprayed directly from aqueous solutions.

Keywords

Glycoside Oligosaccharide Ionization Mass Spectrometry Analytical Mass Analytical Problem 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • M. Karas
    • 1
  • U. Bahr
    • 1
  • T. Dülcks
    • 1
  1. 1.Instrumental Analytical Chemistry, JW Goethe-University, Theodor-Stern-Kai 7, 60590 Frankfurt, GermanyDE

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