Abstract
An improved ELISA for the determination of tobacco mosaic virus (TMV) with linear sweep voltammetry based on a new system of p-aminophenol (PAP)- H2O2- horseradish peroxidase (HRP) has been developed. The enzymatic product 3-[(4-hydroxyphenyl)amino]-4-(2-amino-5-hydroxyphenyl)-6-[(4-hydroxyphenyl)imino]-2,4-cyclohexadiene-1-one, produced from HRP catalyzing the oxidation of PAP with H2O2, yields a sensitive linear sweep voltammetric response at a potential of –0.45 V (vs. SCE) in Britton-Robinson (BR) buffer solution. By using this voltammetric peak, HRP can be measured with a detection limit of 0.4 mU/L and a linear range of 1.0 ∼ 1.0 × 102 mU/ L. The detection limit for the clarified TMV is 4.0 ng/mL and the highest dilution ratio detected for the infected leaf sap is 1 : 3.9 × 106. The processes of the enzyme-catalyzed reaction and the electro-reduction of the product of the enzyme-catalyzed reaction have been investigated.
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Received: 17 November 1998 / Revised: 17 March 1999 / Accepted: 20 March 1999
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Zhang, SS., Jiao, K., Chen, HY. et al. An improved ELISA for the determination of tobacco mosaic virus with linear sweep voltammetry detection based on a new system of PAP-H2O2-HRP. Fresenius J Anal Chem 364, 758–762 (1999). https://doi.org/10.1007/s002160051428
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DOI: https://doi.org/10.1007/s002160051428