Abstract
The adsorptive stripping voltammetric behavior of bovine insulin has been studied by both differential pulse and square-wave techniques, leading to analytical methodologies for its determination in aqueous samples and pharmaceutical preparations. The application of a square-wave mode for the stripping of adsorbed bovine insulin has proved to be much more sensitive, yielding signals 20 times larger than those obtained by applying a differential pulse scan. The precision obtained for the voltammetric method, at a concentration level as low as 0.77 mg L–1 of the protein, was found to be 1.45% (RSD., n = 8) by SWS and 2.20% (RSD., n = 8) by DPS. The pharmaceutical product Actrapid containing biosynthetic human insulin was analyzed by both methods.
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Received: 25 February 1999 / Revised: 6 April 1999 / Accepted: 12 April 1999
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Berzas Nevado, J., Rodriguez Flores, J. & Castañeda Peñalvo, G. Adsorptive stripping square-wave voltammetric behavior of insulin. Fresenius J Anal Chem 364, 753–757 (1999). https://doi.org/10.1007/s002160051427
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DOI: https://doi.org/10.1007/s002160051427