Abstract
Many details of the metabolism and biological significance of trivalent inorganic cations have remained obscure up to now, not least because of the lack of appropriate tools for species analysis of these cations in biological matrices. In order to demonstrate the capabilities of reversed-phase ion-pair chromatography, the distribution of chromium species in brewer’s yeast, previously incubated with radiolabelled 51Cr chloride was investigated. Contradictory to the findings of most other researchers in this area, two low-molecular weight, anionic chromium species were detected in cytosolic yeast extracts. In conclusion, reversed-phase ion-pair chromatography may reveal new details of intracellular metabolism of chromium(III) and, possibly, other trivalent cations.
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Received: 3 August 1998 / Revised: 17 September 1998 / Accepted: 21 September 1998
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Knöchel, A., Weseloh, G. Fractionation of chromium(III) compounds in biological matrices. Fresenius J Anal Chem 363, 533–535 (1999). https://doi.org/10.1007/s002160051241
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DOI: https://doi.org/10.1007/s002160051241