Abstract
A potentiometric method for the determination of oxalate in urine is proposed. It is based on the biochemical reaction catalyzed by oxalate decarboxylase/pervaporation/stopped-flow. The method affords a linear determination range between 0.03 and 0.08 mmol/l and has been successfully applied to the determination of oxalate in urine with excellent agreement of the results with those obtained by the conventional method based on precipitation/dissolution and titration. The effectiveness of the pervaporation process makes sample pretreatment other than dilution unnecessary.
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Received: 20 March 1996 / Revised: 28 May 1996 / Accepted: 31 May 1996
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Cañizares, P., de Castro, M. Enzymatic interference-free assay for oxalate in urine. Fresenius J Anal Chem 357, 777–781 (1997). https://doi.org/10.1007/s002160050248
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DOI: https://doi.org/10.1007/s002160050248