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Fresenius' Journal of Analytical Chemistry

, Volume 368, Issue 8, pp 739–758 | Cite as

Carbohydrate analysis by high-performance anion-exchange chromatography with pulsed amperometric detection: The potential is still growing

  • T. R. I. Cataldi
  • C. Campa
  • G. E. De Benedetto
Review

Abstract

This article reviews recent advances of carbohydrate analysis by high-performance anion-exchange chromatography with pulsed amperometric detection. Starting from the paper of Dennis C. Johnson [1] in which the great analytical promise of such a technique was anticipated, a multitude of exciting new research possibilities have recently emerged. The great attractiveness of high-performance anion-exchange chromatography is largely due to its compatibility with such a sensitive, selective and reliable detection method as pulsed amperometry. This very good match between liquid chromatography and electrochemical detection has allowed the determination of carbohydrates in a variety of complex matrices, for instance, foods, beverages, diary and biotechnological products, vegetal tissues, and also in the area of clinical diagnostics. For this reason, the introduction of HPAEC-PAD into regulated methods is becoming increasingly accepted. A comprehensive collection of applications to carbohydrates and samples of interest is given, with special focus on the separation of closely related sugar compounds using dilute alkaline eluents. Advances in pulsed potential waveforms are also discussed, and a comparison with other liquid chromatographic methods addressed.

Keywords

Electrochemical Detection Vegetal Tissue Regulate Method Reliable Detection Liquid Chromatographic Method 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • T. R. I. Cataldi
    • 1
  • C. Campa
    • 1
  • G. E. De Benedetto
    • 1
  1. 1.Dipartimento di Chimica, Università degli Studi della Basilicata, Via N. Sauro, 85-85100 Potenza, Italy e-mail: cataldi@unibas.itIT

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