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Miniaturized matrix solid-phase dispersion assisted by deep eutectic solvent for acrylamide determination in bread samples

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Abstract

Acrylamide determination is important to state its quantity in baked food preventing any potential carcinogenic effects. Matrix solid-phase dispersion (MSPD) extraction is an extraction procedure based on a homogenization phase between a solid sample and a solid dispersing material to break sample increasing analyte extraction yield, often used for acrylamide determination. The addition of a green deep eutectic solvent (DES) during the MSPD homogenization phase improves the analyte extraction, giving the possibility to reduce the amount of organic solvent used. In this work, a miniaturized MSPD extraction assisted by a DES was developed to determine acrylamide in bread, using high-performance liquid chromatography coupled with mass spectrometry detection. The optimized procedure provides 1:1 (w/w) matrix-to-dispersing material ratio, 2 mL of methanol as extraction solvent, and 50 μL of choline chloride-glycerol DES added during the homogenization phase. Method validation ensured good results with minimum recoveries of 90%, high precision with a maximum intra-day error of 4%, and inter-day error of 6%. Limit of detection and limit of quantification resulted to be 16 μg/kg and 35 μg/kg, respectively. This miniaturized extraction procedure represents a good alternative to those reported in the literature, guaranteeing great performance and respecting green chemistry principles.

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All data generated or analyzed during this study are included in this published article.

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Funding

This study was funded by PRO-FORNO project (n. PROT. A0375-2020–36570) of Regione Lazio, Italy, Avviso Pubblico “Gruppi di ricerca 2020”—POR FESR Lazio 2014–2020—Azione 1.2.

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Authors and Affiliations

  1. Department of Science and Technology for Sustainable Development and One Health, Campus Bio-Medico University of Rome, Via Alvaro del Portillo 21, 00128, Rome, Italy

    Susanna Della Posta, Anna Maria Ascrizzi, Giorgia Pietrangeli, Vittoria Terrigno, Elisa De Arcangelis & Chiara Fanali

  2. Council for Agricultural Research and Economics (CREA), Research Centre for Food and Nutrition, Via Ardeatina 546, 00178, Rome, Italy

    Stefania Ruggeri

Authors
  1. Susanna Della Posta
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  2. Anna Maria Ascrizzi
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  3. Giorgia Pietrangeli
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  5. Elisa De Arcangelis
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  6. Stefania Ruggeri
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  7. Chiara Fanali
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Contributions

Susanna Della Posta (data collection, analysis, writing—original draft preparation), Anna Maria Ascrizzi (material preparation, data collection, analysis, draft), Giorgia Pietrangeli (analysis, draft), Vittoria Terrigno (draft), Elisa De Arcangelis (review and editing), Stefania Ruggeri (review and editing), Chiara Fanali (supervision, conceptualization, writing—original draft preparation).

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Correspondence to Chiara Fanali.

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Published in the topical collection featuring Sustainability in Sample Preparation with guest editors Soledad Cárdenas and Pablo Richter.

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Della Posta, S., Ascrizzi, A.M., Pietrangeli, G. et al. Miniaturized matrix solid-phase dispersion assisted by deep eutectic solvent for acrylamide determination in bread samples. Anal Bioanal Chem (2024). https://doi.org/10.1007/s00216-024-05315-8

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  • DOI: https://doi.org/10.1007/s00216-024-05315-8

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