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Optical fiber surface plasmon resonance sensor using electroless-plated gold film for thrombin detection

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Abstract

This paper describes the simple and label-free detection of thrombin using optical fiber surface plasmon resonance (SPR) sensors based on gold films prepared by the cost-effective method of electroless plating. The plating conditions for simultaneously obtaining gold film on cylindrical core and end surfaces of an optical fiber suitable for measurement were optimized. The fabricated sensor exhibited a linear refractive index sensitivity of 2150 nm/RIU and 7.136 (a.u.)/RIU in the refractive index of 1.3329–1.3605 interrogated by resonance wavelength and amplitude methods respectively and a single wavelength monitoring method was proposed to investigate the sensing performance of this sensor. Polyadenine diblock and thiolated thrombin aptamers were immobilized on gold nanoparticles and gold films respectively to implement a sandwich optical fiber assay for thrombin. The developed optical fiber SPR sensors were successfully used in the determination of thrombin down to 0.56 nM over a wide range from 2 to 100 nM and showed good selectivity for thrombin, which indicated their potential clinical applications for biomedical samples.

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Funding

This work was supported by the Project of Science and Technology Research Program of Chongqing Municipal Education Commission (Grant No. KJQN202200431), the Natural Science Foundation of Chongqing (grant no. cstc2020jcyj-msxmX0792 and CSTB2023NSCQ-MSX0175), Chongqing Medical Scientific Research Project (Joint Project of Chongqing Health Commission and Science and Technology Bureau, 2024QNXM037), Venture and Innovation Support Program for Chongqing Overseas Returnees (No. cx2023030), and the Talents Project of University-Town Hospital of Chongqing Medical University.

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Author notes

  1. Yu Huang and Xiaoyin Pu contributed equally to this work.

Authors and Affiliations

  1. Chongqing Key Laboratory of Multi-scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, 400714, China

    Yu Huang & Xiaoyin Pu

  2. Chongqing School, University of Chinese Academy of Sciences, Chongqing, 400714, China

    Yu Huang & Xiaoyin Pu

  3. Department of Laboratory Medicine, The Affiliated Hospital of Guizhou Medical University, Guiyang, 550004, Guizhou, China

    Husun Qian

  4. Department of Opto-Electronic Engineering, National Dong Hwa University, Hualien, 97401, Taiwan, China

    Chin-Jung Chuang

  5. Department of Clinical Laboratory, Medical Sciences Research Center, University-Town Hospital of Chongqing Medical University, Chongqing, 401331, China

    Shanshan Dong, Jiangling Wu & Jianjiang Xue

  6. The Center for Clinical Molecular Medical Detection, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China

    Wei Cheng

  7. Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, China

    Shijia Ding

  8. Clinical Laboratory, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, 300000, China

    Shengqiang Li

  9. National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin, 300000, China

    Shengqiang Li

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  1. Yu Huang
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Contributions

Yu Huang: conceptualization, writing — original draft, review and editing

Xiaoyin Pu: investigation, writing — original draft

Husun Qian: methodology

Chin-Jung Chuang: formal analysis

Shanshan Dong: validation

Jiangling Wu: project administration, writing — original draft

Jianjiang Xue: data curation

Wei Cheng: validation

Shijia Ding: validation

Shengqiang Li: supervision

Corresponding authors

Correspondence to Yu Huang, Jiangling Wu or Shengqiang Li.

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Huang, Y., Pu, X., Qian, H. et al. Optical fiber surface plasmon resonance sensor using electroless-plated gold film for thrombin detection. Anal Bioanal Chem 416, 1469–1483 (2024). https://doi.org/10.1007/s00216-024-05150-x

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