Abstract
With the expansion of ICP-MS application into the field of bioanalysis, there is an urgent need for novel element tags today. Here, we report the design of a dual-element Ir-Eu tag, opening the door to simultaneous fluorescent imaging and ICP-MS quantification. The ratio of 153Eu/193Ir may serve as a precision control of the labeling process, allowing internal validation of the quantitative results obtained. As for SIRPα and its host cell analysis exemplified here, the Ir-Eu tag demonstrated superior figures of ICP-MS quantification with the LOD (3σ) down to 0.5 (153Eu) and 1.1 (193Ir) pM SIRPα and 220 (153Eu) and 830 (193Ir) RAW264.7 cells more than 130 times more sensitive compared with the LOD (3σ) of 65.2 pM SIRPα at 612 nm using fluorometry. Not limited to these demonstrations, we believe that the design ideas of the dual Ir-Eu tags should be applicable to various cases of bioanalysis when dual optical profiling and ICP-MS quantification are indispensable.
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Funding
We thank the financial supports from the National Natural Science Foundation of China (22193053, 21535007, and 22074127) and the National Key Research and Development Program of China (2022YFF0710202) as well as Medical and Health Key Project of Xiamen (3502Z20191106).
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Sixty-five blood samples from 27 liver cancer patients, 12 lithiasis patients and 26 inflammation patients were provided by Xiamen University affiliated Zhongshan Hospital and permitted by Medical Ethics Council.
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Informed consent was given by the patients donating the blood sample for analysis.
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The authors declare that they have no competing interests. Qiuquan Wang is co-editor of Analytical and Bioanalytical Chemistry but was not involved in the peer review of this paper.
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Published in the topical collection Elemental Mass Spectrometry for Bioanalysis with guest editors Jörg Bettmer, Mario Corte-Rodríguez, and Márcia Foster Mesko.
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Liu, C., Li, P., Yan, X. et al. Design of a dual Ir-Eu tag for fluorescent visualization and ICP-MS quantification of SIRPα and its host cells. Anal Bioanal Chem 416, 2691–2697 (2024). https://doi.org/10.1007/s00216-023-05108-5
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DOI: https://doi.org/10.1007/s00216-023-05108-5