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Copper formate–lysine nanoparticles with polyphenol oxidase-like activity for the detection of epinephrine

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Abstract

Laccase is an enzyme known for its eco-friendly uses in environmental cleanup and biotechnology. However, it has limitations such as low stability, high cost, and complex recycling. So, there is a need for laccase mimics that can effectively imitate its properties. Herein, we created copper formate–lysine nanoparticles (Cuf-Lys) that mimic laccase’s activity. The developed Cuf-Lys demonstrated remarkable polyphenol oxidase-like activity, stability, and recyclability, making them suitable for the fabrication of efficient colorimetric sensors for the detection of epinephrine. These sensors had a specific response and could accurately measure epinephrine concentrations ranging from 2.5 to 50 μM, with a detection limit as low as 1 μM. Furthermore, the biosensor demonstrated high sensitivity and selectivity when applied to the detection of rutin. The limit of detection for rutin was determined to be 0.16 μM while in the linear concentration range of 0.25 to 150.0 μM. We believe that Cuf-Lys provide a new route for the design of laccase mimics, showing potential applications for biomedical diagnosis and environmental monitoring.

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Funding

This work was supported by the National Natural Science Foundation of China (Grant No. 21905237 and 82302275), the National Key Research and Development (R&D) Program of China (No. 2022YFB3805604), the Fundamental Research Funds for the Central Universities (WUT: 2022IVA160 and 2022IVA089), the Young Top-notch Talent Cultivation Program of Hubei Province (Grant No. 40129014), and the 7th Yong elite scientist sponsorship program by CAST (No. YESS20210191).

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  1. State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, School of Chemistry, Chemical Engineering and Life Science, Wuhan University of Technology, Wuhan, 430070, China

    Ping Sun, Yue Zhou, Tong Qiu & Jian Peng

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  1. Ping Sun
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Sun, P., Zhou, Y., Qiu, T. et al. Copper formate–lysine nanoparticles with polyphenol oxidase-like activity for the detection of epinephrine. Anal Bioanal Chem (2023). https://doi.org/10.1007/s00216-023-05095-7

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