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Development, validation, and implementation of an ultratrace analysis method for the determination of moenomycin A, in aquatic animal products

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Abstract

Moenomycin A, an antimicrobial growth promoter widely used as an additive in aquaculture feedstuffs, has been restricted for use in the European Union and China due to its potential risk of promoting resistant strains of pathogenic bacteria and causing residues in aquatic animal products. Although methods for analyzing moenomycin A in feedstuffs have been developed, no established method exists for aquatic matrices. In this study, we present, for the first time, a sensitive and validated high-performance liquid chromatography-tandem mass spectrometry (HPLC–MS/MS) method for the determination of moenomycin A in aquatic animal products. Samples were extracted using methanol and purified with the QuEChERS method employing C18 sorbent. The aliquot was dried under a nitrogen stream, reconstituted with methanol-water solvent, and analyzed by HPLC–MS/MS. The developed method exhibited good linearity (r2 > 0.995) over a wide concentration range (1–100 μg/L) and a low limit of detection (1 µg/kg). Average recoveries ranged between 70 and 110% at spiked concentrations of 1, 50, and 100 μg/kg, with associated intra- and inter-day relative standard deviations of 1.25 to 7.32% (n = 6) and 2.91 to 10.08% (n = 3), for different representative aquatic animal production, respectively. To the best of our knowledge, this is the first reported HPLC–MS/MS method for the quantification of moenomycin A in aquatic animal products. The new approach was effectively employed in the analysis of moenomycin A across various aquatic samples.

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Data availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Funding

This work received financial support from the 2019 Agricultural National Standard Development Project (SCB-20023), and the Central Public-interest Scientific Institution Basal Research Fund, ECSFR, CAFS (2018T02).

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Authors and Affiliations

  1. East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Jungong 300, Shanghai, 200090, People’s Republic of China

    Yunyu Tang, Guangxin Yang, Dongmei Huang, Xuan Zhang, Siman Li, Yongfu Shi & Cong Kong

  2. Shanghai Center of Agri-Product Quality and Safety, Xinfu Middle Road 1528, No.28, Shanghai, 201708, People’s Republic of China

    Yingqing Ma & Weiyi Zhang

  3. Institute of Quality Standard and Testing Technology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, People’s Republic of China

    Wenlei Zhai

  4. Laboratory of Constitution and Reaction of Matter, UFR SSMT, Université Felix Houphouet Boigny, 22 BP 582 Abidjan 22, Abidjan, Côte d’Ivoire

    Essy Kouadio Fodjo

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  1. Yunyu Tang
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Contributions

Conceptualization: Dongmei Huang, Weiyi Zhang, and Cong Kong. Methodology: Yunyu Tang, Guangxin Yang. Formal analysis and investigation: Yunyu Tang, Yingqing Ma, and Guangxin Yang. Validation: Xuan Zhang, Siman Li, and Weiyi Zhang. Visualization: Dongmei Huang. Writing — original draft preparation: Yunyu Tang. Writing — review and editing: Cong Kong, Essy Kouadio Fodjo, and Wenlei Zhai. Funding acquisition: Dongmei Huang. Resources: Dongmei Huang and Cong Kong. Supervision: Cong Kong and Yongfu Shi.

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Correspondence to Dongmei Huang, Weiyi Zhang or Cong Kong.

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Published in the topical collection Food Safety Analysis 2.0 with guest editor Steven J. Lehotay.

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Tang, Y., Yang, G., Ma, Y. et al. Development, validation, and implementation of an ultratrace analysis method for the determination of moenomycin A, in aquatic animal products. Anal Bioanal Chem 416, 745–757 (2024). https://doi.org/10.1007/s00216-023-04965-4

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