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Cu-MOF derived CuO@g-C3N4 nanozyme for cascade catalytic colorimetric sensing

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A Correction to this article was published on 15 August 2023

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Abstract

The use of peroxidase mimics has great potential for various real applications due to their strong catalytic activity. Herein, a facile strategy was proposed to directly prepare CuO@g-C3N4 by Cu-MOF derivatization and demonstrated its efficacy in constructing a multiple enzymatic cascade system by loading protein enzymes onto it. The resulting CuO@g-C3N4 possessed high peroxidase-like activity, with a Michaelis constant (Km) of 0.25 and 0.16 mM for H2O2 and 3,3’,5,5’-tetramethylbenzidine (TMB), respectively. Additionally, the high surface area of CuO@g-C3N4 facilitated the loading of protein enzymes and maintained their activity over an extended period, expanding the potential applications of CuO@g-C3N4. To test its feasibility, CuO@g-C3N4/protein oxidase complex was prepared and used to sense the ripeness and freshness of fruits and meat, respectively. The mechanism relied on the fact that the ripeness of fruits increased and freshness of food decreased with the release of marked targets, such as glucose and xanthine, which could produce H2O2 when digested by the corresponding oxidase. The peroxidase mimics of CuO@g-C3N4 could then sensitively colorimetric detect H2O2 in present of TMB. The obtained CuO@g-C3N4/oxidase complex exhibited an excellent linear response to glucose or xanthine in the range of 1.0–120 μmol/L or 8.0–350 μmol/L, respectively. Furthermore, accurate quantification of glucose and xanthine in real samples is achieved with spiked recoveries ranging from 80.2% to 120.0% and from 94.2% to 112.0%, respectively. Overall, this work demonstrates the potential of CuO@g-C3N4 in various practical applications, such as food freshness detection.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (Nos. 21964008, 22104025); Guangxi Natural Science Foundation of China (Nos. 2020GXNSFBA159050, 2020GXNSFBA297147).

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  1. Manman Lu and Zhifeng Wang contributed equally to this work.

Authors and Affiliations

  1. Guangxi Key Laboratory of Drug Discovery and Optimization, Guangxi Engineering Research Center for Pharmaceutical Molecular Screening and Druggability Evaluation, School of Pharmacy, Guilin Medical University, Guilin, 541199, People’s Republic of China

    Zhengyi Chen

  2. College of Food and Bioengineering, Hezhou University, Hezhou, 542899, People’s Republic of China

    Manman Lu, Wei Xie, Zhi Zhang, Linjing Su & Yuhao Xiong

  3. Department of Burn, Wound Repair Surgery, and Plastic Surgery, Department of Aesthetic Surgery, Affiliated Hospital of Guilin Medical University, Guilin, 541001, People’s Republic of China

    Zhifeng Wang

  4. Department of Food and Chemical Engineering, Liuzhou Institute of Technology, Liuzhou, 545616, People’s Republic of China

    Manman Lu

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The original online version of this article was revised: Author Zhi Zhang affiliation should be Hezhou University and numbering of affiliations has been revised.

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Lu, M., Wang, Z., Xie, W. et al. Cu-MOF derived CuO@g-C3N4 nanozyme for cascade catalytic colorimetric sensing. Anal Bioanal Chem 415, 5949–5960 (2023). https://doi.org/10.1007/s00216-023-04844-y

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