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Engineering of a novel D–A type fluorophore with hydrogen bond-induced enhanced emission property for sensitively detecting endogenous HOCl in living cells and tissues

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Abstract

Fluorescence imaging has been widely employed for biomedical research and clinical diagnostics. With ease of synthesis and excellent photophysical properties, D–A type fluorophores are widely designed for fluorescence imaging. However, traditional D–A type fluorophores are solvatochromic which reduces the fluorescence brightness in the biological system. To solve this problem and build on our previous work, we devised a novel HIEE fluorophore MTC with typical anti-solvatochromic fluorescence. Furthermore, the activated fluorescent probe designed based on MTC showed excellent imaging performance. We believe that the strategy based on the fluorophores with typical anti-solvatohromic fluorescence can be a useful platform for designing fluorescent probes for high-brightness imaging in the biological system.

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Funding

This work is supported by NSFC (22004033, 21977027, 21877029, 21605091) and Shandong Key Laboratory of Biochemical Analysis (SKLBA2107).

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Author notes

  1. Si-Min Wang and Xiaofeng Lou contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, People’s Republic of China

    Si-Min Wang, Xiaofeng Lou, Shuai Xu, Tian-Bing Ren, Shuang-Yan Huan, Lin Yuan & Xiao-Bing Zhang

  2. Shandong Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China

    Zhi-Ling Song

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  1. Si-Min Wang
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Correspondence to Tian-Bing Ren or Shuang-Yan Huan.

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All animal experiments were performed in accordance with protocol no. SYXK (Xiang) 2008–0001 approved by the Laboratory Animal Center of Hunan.

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Published in the topical collection Young Investigators in (Bio-)Analytical Chemistry 2023 with guest editors Zhi-Yuan Gu, Beatriz Jurado-Sánchez, Thomas H. Linz, Leandro Wang Hantao, Nongnoot Wongkaew, and Peng Wu.

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Wang, SM., Lou, X., Xu, S. et al. Engineering of a novel D–A type fluorophore with hydrogen bond-induced enhanced emission property for sensitively detecting endogenous HOCl in living cells and tissues. Anal Bioanal Chem 415, 4185–4196 (2023). https://doi.org/10.1007/s00216-023-04550-9

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  • DOI: https://doi.org/10.1007/s00216-023-04550-9

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