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Electrochemical ELASA: improving early cancer detection and monitoring

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Abstract

The discovery of new molecular biomarkers of cancer during the last decades and the development of new diagnostic devices exploiting those have significantly contributed to the clinical analysis of cancer and to improve the outcomes. Among those, liquid biopsy sensors exploiting aptamers for the detection of cancer biomarkers in body fluids are useful and accurate tools for a fast and inexpensive non-invasive screening of population. The incorporation of aptamers in electrochemical sandwich biosensors using enzyme labels, a so-called ELASA, has demonstrated its utility to improve the detection schemes. In this review, we overview the existing ELASA assays for numerous cancer biomarkers as alternatives to the traditional ELISA and discuss their possibilities to reach the market, currently dominated by optical immunoassays.

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Funding

The work was funded by the Novo Nordisk Foundation through the project “Validating Serum Tests for Human Epidermal growth factor Receptor-2 for Precise Diagnosis and Stratification of Breast and Gastro-Oesophagal Cancers”; the grant reference number NNF20OC0065428.

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  1. Interdisciplinary Nanoscience Center (iNANO), Faculty of Natural Sciences, Aarhus University, Gustav Wieds Vej 14, 8000, Aarhus C, Denmark

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Published in the topical collection Young Investigators in (Bio-)Analytical Chemistry 2023 with guest editors Zhi-Yuan Gu, Beatriz Jurado-Sánchez, Thomas H. Linz, Leandro Wang Hantao, Nongnoot Wongkaew, and Peng Wu.

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Díaz-Fernández, A., Ferapontova, E.E. Electrochemical ELASA: improving early cancer detection and monitoring. Anal Bioanal Chem 415, 3831–3846 (2023). https://doi.org/10.1007/s00216-023-04546-5

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