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Comprehensive lipidomic profiling by plasma separation cards

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Abstract

Large-scale lipidomic analyses have been limited by the cost and accessibility of traditional venipuncture sampling. Microsampling techniques offer a less-invasive and more accessible alternative. From a single drop of blood, plasma separation cards (PSC) deliver two volumetric dried plasma samples which are studied here for profiling endogenous blood lipids. Six lots of EDTA-treated human whole blood were used to compare PSC, dried blood spot analyses (DBS), and classic wet plasma extractions. Six replicate extractions were performed for each lot. Nontargeted lipidomics was performed by liquid chromatography-high resolution tandem mass spectrometry. Lipids were annotated by accurate mass/retention time matching and MS/MS spectral library matching using peak intensities for quantitation. Four hundred ninety-eight compounds covering 24 lipid subclasses were annotated. Inter-lot repeatability was evaluated by the percent relative standard deviation (%RSD) for each lot, giving median %RSD values across the lots at 14.6% for PSC, 9.3% for DBS, and 8.6% for wet plasma. Strong correlations of lipid peak intensities between wet plasma and PSCs were observed, but less for DBS. Lipid recovery and stability were comparable between the PSC and DBS samples, with roughly 60% of annotated lipids stable at room temperature after 28 days. Overall, PSCs provide a better alternative for quantitative blood lipidomic analyses compared to dried blood spots. However, problems with lipid stability for samples handled and shipped at room temperature are currently unavoidable outside of a clinical setting. Data transferability and comparability to standard plasma is lipid and lipid class dependent.

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Acknowledgements

The authors would like to thank Emily Olson and Michele Mietus-Snyder of Children’s National Hospital for their contributions to the initial validation experiments.

Funding

This study was supported by the National Institutes of Health grants U19 AG023122 and R01HL157535.

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Authors and Affiliations

  1. Department of Chemistry, University of California Davis, Davis, CA, USA

    Lauren M. Bishop & Oliver Fiehn

  2. West Coast Metabolomics Center, University of California Davis, Davis, CA, USA

    Lauren M. Bishop & Oliver Fiehn

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Correspondence to Oliver Fiehn.

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Bishop, L.M., Fiehn, O. Comprehensive lipidomic profiling by plasma separation cards. Anal Bioanal Chem 415, 193–201 (2023). https://doi.org/10.1007/s00216-022-04399-4

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