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Ag nanozyme strengthened by folic acid: Superior peroxidase-mimicking activity and application for visual monitoring of dopamine

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Abstract

Dopamine (DA) is an important neurotransmitter; however, any excess or deficiency of DA will cause several diseases in humans. To monitor DA efficiently and conveniently, a Ag nanozyme strengthened by bioactive folic acid (FA@AgNPs) was developed by homogeneous redox assembly. After the microstructure and performance were characterized in detail, it was noted that the proposed FA@AgNPs possessed superior peroxidase-like activity due to the ultra-small Ag nanoparticles and multiple amino, hydroxyl, and aromatic rings in FA. FA@AgNPs accelerated the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) with a low Michaelis constant (Km) and high maximal reaction rate (Vmax). Importantly, the characteristic absorbance intensity of FA@AgNPs-TMB-H2O2 at 652 nm (A652) was exclusively deteriorated in the presence of trace DA, accompanied by a visual color change from blue to colorless. Under the optimized conditions (pH 4.0, 300 μL 1.5 mM TMB, 300 μL 1.0 M H2O2 and incubated for 30 min at room temperature), there expressed an excellent linear relationship between lgA0/A652 and cDA from 1.0 ×10−8 to 6.67×10−6 mol/L with a low limit detection of 7.1×10−10 mol/L (S/N=3). When applied for monitoring of DA in real fruit juice and pharmaceutical samples, the recovery was between 96.6% and 104.9%, with RSD less than 2.2%. The enhanced peroxidase-like activity of the FA@AgNP system and its selective recognition mechanism for DA are also proposed.

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Funding

This work was supported by the Natural Science Foundation of Shandong Province (Nos: ZR202102210020 and ZR2018MB038) and the National Natural Science Foundation of China (Nos: 21806089).

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering & Shandong Key Laboratory of Life-Organic Analysis, Qufu Normal University, Qufu, 273165, China

    Yulian Tang, Xue Lv, Wenxin Gou, Xuemei Zhou, Junkai Hao, Jing Feng, Yuji Qi, Lei Hu & Zhengquan Yan

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  1. Yulian Tang
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Correspondence to Lei Hu or Zhengquan Yan.

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Tang, Y., Lv, X., Gou, W. et al. Ag nanozyme strengthened by folic acid: Superior peroxidase-mimicking activity and application for visual monitoring of dopamine. Anal Bioanal Chem 414, 6611–6620 (2022). https://doi.org/10.1007/s00216-022-04222-0

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