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A dual-signal sensing strategy based on ratiometric fluorescence and colorimetry for determination of Cu2+ and glyphosate

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Abstract

Herein, a dual-signal sensing strategy based on ratiometric fluorescence and colorimetry for Cu2+ and glyphosate determination was constructed. Fluorescence silicon nanoparticles (SiNPs) were prepared by hydrothermal reaction, which has maximum fluorescence intensity under the excitation of 355 nm. o-Phenylenediamine (OPD) was oxidized through Cu2+ to generate 2,3-diaminophenazine (oxOPD). The obtained oxOPD showed a strong absorption peak at 417 nm and quenched the fluorescence of SiNPs at 446 nm due to fluorescence resonance energy transfer (FRET). Meanwhile, oxOPD produced a new fluorescence emission at 556 nm forming a ratiometric state. With increasing Cu2+, the original solution changed from colorless to yellow. When glyphosate was present, the interaction between Cu2+ and the functional groups of glyphosate could reduce the oxidation of oxOPD, resulting in the enhancement of fluorescence at 446 nm and the decrease of fluorescence at 556 nm. Furthermore, the addition of glyphosate changed yellow solution to colorless. Under the optimal conditions of OPD (1 mM), 20 mM Tris–HCl buffer (pH 7.5), and incubation time (4 h), the ratiometric fluorescence sensor had good selectivity and showed a wide linear range of 0.025–20 μM with the LOD of 0.008 μM for Cu2+ and 0.15–1.5 μg/mL with the LOD of 0.003 μg/mL for glyphosate, respectively. Besides, it is worth mentioning that this developed sensing system showed good performance in real samples, providing a simple and reliable dual-signal detection strategy.

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Acknowledgements

The authors thank the Analytical and Testing Center of Chongqing University for TEM/FT-IR/XRD/XPS and the sharing fund of Chongqing University’s large equipment.

Funding

This work was supported by the National Key R&D Program of China (2017YFC1602000), National Natural Science Foundation of China (No. 31171684), Graduate Scientific Research and Innovation Foundation of Chongqing, China (Grant CYS21069), Key Laboratory of Wuliangye-Flavor Liquor Solid-State Fermentation, China National Light Industry (2021JJ001), Key Laboratory of Brewing Molecular Engineering of China Light Industry (BME-202108), Brew Microorganisms Technology and Application of Key Laboratory Project in Sichuan Province (NJ2020-03), Chongqing Graduate Tutor Team Construction Project, and the Natural Science Foundations of Hainan Province (No. 219QN290).

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Authors and Affiliations

  1. Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing, 400044, People’s Republic of China

    Yixia Yang, Li Li, Xianfeng Wang, Jiawei Li, Huan Liu, Xiaofang Liu, Danqun Huo & Changjun Hou

  2. Agricultural Products Processing Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, 524001, Guangdong, China

    Liyun Lin

  3. Chongqing Institute for Food and Drug Control, Chongqing, 401121, People’s Republic of China

    Huan Liu

  4. Chongqing Key Laboratory of Bio-Perception & Intelligent Information Processing, School of Microelectronics and Communication Engineering, Chongqing University, Chongqing, 400044, People’s Republic of China

    Changjun Hou

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  1. Yixia Yang
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  2. Li Li
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  3. Liyun Lin
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Yang, Y., Li, L., Lin, L. et al. A dual-signal sensing strategy based on ratiometric fluorescence and colorimetry for determination of Cu2+ and glyphosate. Anal Bioanal Chem 414, 2619–2628 (2022). https://doi.org/10.1007/s00216-022-03898-8

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