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A green and facile approach to a graphene-based peroxidase-like nanozyme and its application in sensitive colorimetric detection of l-cysteine


A facile and green approach to the preparation of peroxidase-like nanozymes by reducing and functionalizing graphene oxide (rGO) with Ganoderma polysaccharide (GP) has been achieved in this work. Our results showed that the as-fabricated nanozyme, namely rGO-GP, possessed the excellent property of simulating peroxidase with higher catalytic activity compared with GO or rGO obtained by using chitosan, which may be due to the better dispersion of rGO-GP in the solution. Steady-state kinetics studies further showed that the catalytic process conformed to Michaelis-Menten equation and ping-pong mechanism. Benefiting from the excellent peroxidase property of rGO-GP, we have also successfully established a highly sensitive and selective colorimetric detection approach to trace detection of l-cysteine (l-Cys). The limit of detection (LOD) of l-cysteine is 0.1 μM and the linear detection range is 2–30 μM. Furthermore, the nanozyme was successfully applied for detecting l-cysteine in serum. This work therefore demonstrates the advantages of rGO-GP as an effective nanozyme in both its green synthesis and detecting application.

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This work was financially supported by the Anhui Provincial Key Research and Development Plans (202004i07020014, 1704a0902017) and National Natural Science Foundation of China (11635013).

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Correspondence to Qing Huang.

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Liu, C., Zhao, Y., Xu, D. et al. A green and facile approach to a graphene-based peroxidase-like nanozyme and its application in sensitive colorimetric detection of l-cysteine. Anal Bioanal Chem 413, 4013–4022 (2021).

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  • Nanozymes
  • Graphene oxide (GO)
  • Reduced graphene oxide (rGO)
  • Green synthesis
  • Ganoderma polysaccharide (GP)
  • l-Cysteine