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A green and facile approach to a graphene-based peroxidase-like nanozyme and its application in sensitive colorimetric detection of l-cysteine

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Abstract

A facile and green approach to the preparation of peroxidase-like nanozymes by reducing and functionalizing graphene oxide (rGO) with Ganoderma polysaccharide (GP) has been achieved in this work. Our results showed that the as-fabricated nanozyme, namely rGO-GP, possessed the excellent property of simulating peroxidase with higher catalytic activity compared with GO or rGO obtained by using chitosan, which may be due to the better dispersion of rGO-GP in the solution. Steady-state kinetics studies further showed that the catalytic process conformed to Michaelis-Menten equation and ping-pong mechanism. Benefiting from the excellent peroxidase property of rGO-GP, we have also successfully established a highly sensitive and selective colorimetric detection approach to trace detection of l-cysteine (l-Cys). The limit of detection (LOD) of l-cysteine is 0.1 μM and the linear detection range is 2–30 μM. Furthermore, the nanozyme was successfully applied for detecting l-cysteine in serum. This work therefore demonstrates the advantages of rGO-GP as an effective nanozyme in both its green synthesis and detecting application.

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Funding

This work was financially supported by the Anhui Provincial Key Research and Development Plans (202004i07020014, 1704a0902017) and National Natural Science Foundation of China (11635013).

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Authors and Affiliations

  1. CAS Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, Anhui Key Laboratory of Environmental Toxicology and Pollution Control Technology, Hefei Institute of IntelligentAgriculture, Institute of Intelligent Machines,Hefei Institutes of Physical Science, Chinese Academy of Sciences (CAS), Hefei, 230031, Anhui, China

    Chao Liu, Yunmeng Zhao, Di Xu, Xinxin Zheng & Qing Huang

  2. Science Island Branch of Graduate School, University of Science and Technology of China, Hefei, 230026, Anhui, China

    Chao Liu, Yunmeng Zhao, Di Xu, Xinxin Zheng & Qing Huang

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  1. Chao Liu
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  2. Yunmeng Zhao
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  3. Di Xu
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  5. Qing Huang
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Correspondence to Qing Huang.

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Liu, C., Zhao, Y., Xu, D. et al. A green and facile approach to a graphene-based peroxidase-like nanozyme and its application in sensitive colorimetric detection of l-cysteine. Anal Bioanal Chem 413, 4013–4022 (2021). https://doi.org/10.1007/s00216-021-03352-1

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