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Green microfluidic liquid-phase microextraction of polar and non-polar acids from urine

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Abstract

In this work, hippuric acid (log P = 0.5), anthranilic acid (log P = 1.3), ketoprofen (log P = 3.6), and naproxen (log P = 3.0) were simultaneously extracted by a green microfluidic device based on the principles of liquid-phase microextraction (LPME). Different deep eutectic solvents (DESs) were investigated as supported liquid membrane (SLM), and a mixture of camphor and menthol as eutectic solvents in the molar ratio 1:1 was found to be highly efficient for the simultaneous extraction of non-polar and polar acidic drugs. LPME was conducted for 6 min per sample. Urine sample was delivered to the system at 1 μL min−1, and target analytes were extracted exhaustively (75–100% recovery) across the DES SLM, and into pure aqueous phosphate buffer pH 11.0 delivered as acceptor at 1 μL min−1. The acceptor was analyzed with liquid chromatography-UV detection. Interestingly, the DES enabled extraction of both the polar and non-polar model analytes at the same time; all chemicals were green and non-hazardous, and the chemical waste was less than 1 mg per sample.

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Acknowledgements

This work was supported by the Agencia de Gestió d’Ajusts Universitaris i the Recerca (2017-SGR-329). Elia Santigosa thanks Universitat Autònoma de Barcelona (UAB) for the PIF fellowship.

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Authors and Affiliations

  1. Department of Analytical Chemistry, Universitat Autónoma de Barcelona, Bellaterra, 08193, Barcelona, Spain

    Elia Santigosa & María Muñoz

  2. Department of Pharmacy, University of Oslo, P.O. Box 1068, Blindern, 0316, Oslo, Norway

    Stig Pedersen-Bjergaard

  3. Department of Analytical Chemistry, Faculty of Chemistry, University of Seville, c/Prof. García González s/n, 41012, Seville, Spain

    María Ramos-Payán

Authors
  1. Elia Santigosa
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  2. Stig Pedersen-Bjergaard
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  3. María Muñoz
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  4. María Ramos-Payán
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Corresponding author

Correspondence to María Ramos-Payán.

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Santigosa, E., Pedersen-Bjergaard, S., Muñoz, M. et al. Green microfluidic liquid-phase microextraction of polar and non-polar acids from urine. Anal Bioanal Chem 413, 3717–3723 (2021). https://doi.org/10.1007/s00216-021-03320-9

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  • DOI: https://doi.org/10.1007/s00216-021-03320-9

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