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Numerical and experimental analysis of a high-throughput blood plasma separator for point-of-care applications

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Blood plasma separation from undiluted blood is an essential step in many diagnostic procedures. This study focuses on the numerical optimization of the microfluidic blood plasma separator (BPS) and experimental validation of the results to achieve portable blood plasma separation with high purity and reasonable yield. The proposed design has two parts: a microchannel for blood processing and a tank below the aforementioned main channel for plasma collection. The study uses 3D computational fluid dynamic analysis to investigate the optimal ratio of heights between the top microchannel and the tank and their geometry at various flow rates. Thereafter, the results are compared with the experimental findings of the fabricated devices. These results are contrasted with some recent reported works to verify the proposed device’s contribution to the improvement in the quality and quantity of the extracted plasma. The optimized design is capable of achieving a 19% yield with purity of 77.1%, depending on the requirement of the point-of-care (POC) application. These amounts could be tuned, for instance to 100% pure plasma, but the yield would decrease to 9%. In this study, the candidate application is hemostasis; therefore, the BPS is integrated to a biomimetic surface for hemostasis evaluation near the patients.

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This research was funded by the Spanish Ministry of Economy and Competitively, grant numbers CTQ2017-84966-C2-1-R. Red Nacional de Microfluídica. RED2018-102829-T. The authors would like to thank Dr. Escolar and Dr. Mir for their help in hemostasis evaluation.

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Correspondence to Jasmina Casals-Terré.

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The authors declare that there is no conflict of interest.

All co-authors have seen and agree with the content of the manuscript.

The studies were approved by the Comitè d’Ètica de la Universitat Politècnica, under the supervision of the Vice-Dean of Research. The studies were performed according to the Declaration of Helsinki; therefore, all individual participants signed informed consent forms.

All individual participant blood samples were obtained from the Catalan Blood and Tissue Bank.

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Karimi, S., Mojaddam, M., Majidi, S. et al. Numerical and experimental analysis of a high-throughput blood plasma separator for point-of-care applications. Anal Bioanal Chem 413, 2867–2878 (2021).

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