Skip to main content
Log in

Numerical and experimental analysis of a high-throughput blood plasma separator for point-of-care applications

  • Research Paper
  • Published:
Analytical and Bioanalytical Chemistry Aims and scope Submit manuscript

Abstract

Blood plasma separation from undiluted blood is an essential step in many diagnostic procedures. This study focuses on the numerical optimization of the microfluidic blood plasma separator (BPS) and experimental validation of the results to achieve portable blood plasma separation with high purity and reasonable yield. The proposed design has two parts: a microchannel for blood processing and a tank below the aforementioned main channel for plasma collection. The study uses 3D computational fluid dynamic analysis to investigate the optimal ratio of heights between the top microchannel and the tank and their geometry at various flow rates. Thereafter, the results are compared with the experimental findings of the fabricated devices. These results are contrasted with some recent reported works to verify the proposed device’s contribution to the improvement in the quality and quantity of the extracted plasma. The optimized design is capable of achieving a 19% yield with purity of 77.1%, depending on the requirement of the point-of-care (POC) application. These amounts could be tuned, for instance to 100% pure plasma, but the yield would decrease to 9%. In this study, the candidate application is hemostasis; therefore, the BPS is integrated to a biomimetic surface for hemostasis evaluation near the patients.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Carboni EJ. The Margination and Transport of Particles in Blood Flow, Degree Thesis, University of Connecticut, 2017.

  2. Kim P, Ong EH, Li KH, Yoon YJ, Ng SH, Puttachat K. Low-cost, disposable microfluidics device for blood plasma extraction using continuously alternating paramagnetic and diamagnetic capture modes. Biomicrofluidics. 2016 Mar 17;10(2):024110.

    Article  Google Scholar 

  3. Tripathi S, Kumar YB, Agrawal A, Prabhakar A, Joshi SS. Microdevice for plasma separation from whole human blood using bio-physical and geometrical effects. Sci Rep. 2016 Jun 9;6:26749.

    Article  CAS  Google Scholar 

  4. Noruz shamsian O, Mohseni A, Mojaddam M. Design of a Micro-Separator for Circulating Tumor Cells (CTCs) from Blood Flow Using Hybrid Pinched Flow Fractionation (PFF) and Dielectrophoresis Methods. J Fluid Mech. 2020;10(1):281–96.

    Google Scholar 

  5. Xiang N, Ni Z. High-throughput blood cell focusing and plasma isolation using spiral inertial microfluidic devices. Biomed Microdevices. 2015;17(6):110.

    Article  Google Scholar 

  6. Madadi H, Casals-Terré J, Mohammadi M. Self-driven filter-based blood plasma separator microfluidic chip for point-of-care testing. Biofabrication. 2015;7(2):025007.

    Article  Google Scholar 

  7. Mohammadi M, Madadi H, Casals-Terré J, Sellarès J. Hydrodynamic and direct-current insulator-based dielectrophoresis (H-DC-iDEP) microfluidic blood plasma separation. Anal Bioanal Chem. 2015;407(16):4733–44.

    Article  CAS  Google Scholar 

  8. Yang S, Ündar A, Zahn JD. A microfluidic device for continuous, real time blood plasma separation. Lab Chip. 2006;6(7):871–80.

    Article  CAS  Google Scholar 

  9. Kim B, Choi S. Smart pipette and microfluidic pipette tip for blood plasma separation. Small. 2016;12(2):190–7.

    Article  CAS  Google Scholar 

  10. Shatova TA, Lathwal S, Engle MR, Sikes HD, Jensen KF. Portable, constriction–expansion blood plasma separation and polymerization-based malaria detection. Anal Chem. 2016;88(15):7627–32.

    Article  CAS  Google Scholar 

  11. Kim B, Oh S, You D, Choi S. Microfluidic pipette tip for high-purity and high-throughput blood plasma separation from whole blood. Anal Chem. 2017;89(3):1439–44.

    Article  CAS  Google Scholar 

  12. Kersaudy-Kerhoas M, Kavanagh DM, Dhariwal RS, Campbell CJ, Desmulliez MP. Validation of a blood plasma separation system by biomarker detection. Lab Chip. 2010;10(12):1587–95.

    Article  CAS  Google Scholar 

  13. Prabhakar A, Kumar YB, Tripathi S. Agrawal A. A novel, compact and efficient microchannel arrangement with multiple hydrodynamic effects for blood plasma separation. Microfluid Nanofluid. 2015 May 1;18(5-6):995–1006.

    Article  CAS  Google Scholar 

  14. Li Z, Li X, McCracken B, Shao Y, Ward K, Fu J. A miniaturized hemoretractometer for blood clot retraction testing. Small. 2016;12(29):3926–34.

    Article  CAS  Google Scholar 

  15. Fowler A, Perry DJ. Laboratory monitoring of haemostasis. Anaesthesia. 2015;70:68–e24.

    Article  Google Scholar 

  16. Santin M, Phillips G. History of biomimetic, bioactive, and bioresponsive biomaterials. Biomimetic, Bioresponsive, and Bioactive Materials: An Introduction to Integrating Materials with Tissues. 2012:1.

  17. Jigar Panchal H, Kent NJ, Knox AJ, Harris LF. Microfluidics in haemostasis: A review. Molecules. 2020;25(4):833.

    Article  Google Scholar 

  18. Hickman DA, Pawlowski CL, Sekhon UD, Marks J, Gupta AS. Biomaterials and advanced technologies for hemostatic management of bleeding. J Adv Mater. 2018 Jan;30(4):1700859.

    Article  Google Scholar 

  19. Cengel YA. Fluid mechanics. Tata McGraw-Hill Education; 2010.

  20. Karimi S, Mehrdel P, Farré-Lladós J. Casals-Terré J. A passive portable microfluidic blood–plasma separator for simultaneous determination of direct and indirect ABO/Rh blood typing. Lab Chip. 2019;19(19):3249–60.

    Article  CAS  Google Scholar 

  21. Karimi S, Farré-Lladós J, Mir E, Escolar G, Casals-Terré J. Hemostasis-on-a-chip: Impedance spectroscopy meets microfluidics for hemostasis evaluation. Micromachines-Basel. 2019;10(8):534.

    Article  Google Scholar 

  22. Neeves KB, Onasoga AA, Wufsus AR. The use of microfluidics in hemostasis: clinical diagnostics and biomimetic models of vascular injury. Curr. 2013;20(5):417–23.

    CAS  Google Scholar 

  23. Michelson AD. Methods for the measurement of platelet function. Am J Cardiol. 2009;103(3):20A–6A.

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This research was funded by the Spanish Ministry of Economy and Competitively, grant numbers CTQ2017-84966-C2-1-R. Red Nacional de Microfluídica. RED2018-102829-T. The authors would like to thank Dr. Escolar and Dr. Mir for their help in hemostasis evaluation.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Jasmina Casals-Terré.

Ethics declarations

The authors declare that there is no conflict of interest.

All co-authors have seen and agree with the content of the manuscript.

The studies were approved by the Comitè d’Ètica de la Universitat Politècnica, under the supervision of the Vice-Dean of Research. The studies were performed according to the Declaration of Helsinki; therefore, all individual participants signed informed consent forms.

All individual participant blood samples were obtained from the Catalan Blood and Tissue Bank.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

ESM 1

(PDF 665 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Karimi, S., Mojaddam, M., Majidi, S. et al. Numerical and experimental analysis of a high-throughput blood plasma separator for point-of-care applications. Anal Bioanal Chem 413, 2867–2878 (2021). https://doi.org/10.1007/s00216-021-03190-1

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00216-021-03190-1

Keywords

Navigation