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Rapid, user-friendly, and inexpensive detection of azidothymidine

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Abstract

Strict adherence to highly active antiretroviral therapy (HAART) is very important to improve the quality of life for HIV-positive patients to reduce new infections and determine treatment success. Azidothymidine (AZT) is an antiretroviral drug commonly used in HAART treatment. In this research, an “add, mix, and measure” assay was developed to detect AZT within minutes. Three different probes designed to release fluorophores when samples containing AZT are added were synthesized and characterized. The limit of detection to AZT in simulated urine samples was determined to be 4 μM in 5 min for one of the probes. This simple and rapid point-of-care test could potentially be used by clinicians and health care workers to monitor the presence of AZT in low resource settings.

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Data availability

Synthesis of probes, data of fluorescence assays, determination of detection limits of AZT by probes, and characterization of all compounds and intermediates are provided in the ESM.

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Funding

This study was financially supported by the National Institute of Allergy and Infectious Diseases (Grant number 5R61AI140475).

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Author notes

  1. Ying Luo and Tianwei Jia contributed equally to this work.

Authors and Affiliations

  1. 788 Petit Science Center, Department of Chemistry, Center for Diagnostics and Therapeutics, Georgia State University, 161 Jesse Hill Jr. Drive, Atlanta, GA, 30302, USA

    Ying Luo, Tianwei Jia, Jieqiong Fang, Dandan Liu, Varma Saikam, Xiaolin Sheng & Suri S. Iyer

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  1. Ying Luo
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Correspondence to Suri S. Iyer.

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Luo, Y., Jia, T., Fang, J. et al. Rapid, user-friendly, and inexpensive detection of azidothymidine. Anal Bioanal Chem 413, 1999–2006 (2021). https://doi.org/10.1007/s00216-021-03168-z

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  • DOI: https://doi.org/10.1007/s00216-021-03168-z

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