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Analytical combinations to evaluate the macromolecular composition of extracellular substances (ECS) from Lactobacillus plantarum cell culture media

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Abstract

Sugar-enriched media are used to produce extracellular substances (ECS) by Lactobacillus plantarum WCSF1, with a focus on growing stages and carbon source substrates. Combination of size exclusion chromatography and ATR-FTIR spectroscopy provides physicochemical patterns of bulk ECS produced along culture growing time. Secreted biopolymers present polydisperse and high molecular weight distributions, with significant amounts of carbohydrates and proteins. Results, supported by a multivariate statistical analysis, enable to differentiate the macromolecular content of bacterial ECS along the growing stages regardless of the growing media, highlighting a higher production of proteinaceous materials compared to polysaccharides. At the end of the exponential phase, common exoproteins were present in all the tested sugar-enriched media such as transglycosylases between 20 and 35 kDa, a muropeptidase at 36.9 kDa and a cell wall hydrolase. Additionally, L. plantarum WCFS1 secretes ECS with a greater diversity of proteins, when growing in the sucrose-enriched media.

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Acknowledgments

The authors would like to thank the Divva Development Facility, IUVV Dijon, Agrosup Dijon, Univ. Bourgogne Franche-Comté, F-21000 Dijon France and Claire Lhomme (Université de Bourgogne, Dijon, France) for their technical assistance.

Funding

This work was supported by the Regional Council of Bourgogne Franche-Comté and the “Fonds Européen de Développement Régional (FEDER)” [CRB 2016-9201AAO048S01632] and the Ministère de lʼEnseignement supérieur, de la Recherche et de lʼInnovation, France.

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  1. University of Bourgogne Franche-Comté, AgroSup Dijon, PAM UMR A 02.102, F-21000, Dijon, France

    Peio Elichiry-Ortiz, Stéphanie Weidmann, Dominique Champion & Christian Coelho

  2. Clinical Innovation Proteomic Platform – CLIPP, 15, Bd Maréchal de Lattre de Tassigny, Bat. Medecine B3, BP37013, 21070, Dijon, France

    Pauline Maes

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Elichiry-Ortiz, P., Maes, P., Weidmann, S. et al. Analytical combinations to evaluate the macromolecular composition of extracellular substances (ECS) from Lactobacillus plantarum cell culture media. Anal Bioanal Chem 413, 519–531 (2021). https://doi.org/10.1007/s00216-020-03022-8

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