Lipids are biomolecules of crucial importance involved in critical biological functions. Yet, lipid content determination using mass spectrometry is still challenging due to their rich structural diversity. Preferential ionisation of the different lipid species in the positive or negative polarity is common, especially when using soft ionisation mass spectrometry techniques. Here, we demonstrate the potency of a dual-polarity approach using surface-assisted laser desorption/ionisation coupled to Fourier transform-ion cyclotron resonance (SALDI FT-ICR) mass spectrometry imaging (MSI) combined with Kendrick mass defect data filtering to (i) identify the lipids detected in both polarities from the same tissue section and (ii) show the complementarity of the dual-polarity data, both regarding the lipid coverage and the spatial distributions of the various lipids. For this purpose, we imaged the same mouse brain section in the positive and negative ionisation modes, on alternate pixels, in a SALDI FT-ICR MS imaging approach using gold nanoparticles (AuNPs) as dual-polarity nanosubstrates. Our study demonstrates, for the first time, the feasibility of (i) a dual-polarity SALDI-MSI approach on the same tissue section, (ii) using AuNPs as nanosubstrates combined with a FT-ICR mass analyser and (iii) the Kendrick mass defect data filtering applied to SALDI-MSI data. In particular, we show the complementarity in the lipids detected both in a given ionisation mode and in the two different ionisation modes.
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The authors acknowledge Dr. Virginie Bertrand for her technical support in the preparation of mouse brain sections and Mathieu Tiquet for the development of the MSI acquisition method.
Wendy H. Müller and Cedric Malherbe acknowledge support from the F.R.S.-FNRS (Fonds de la Recherche Scientifique - FNRS) as Research Fellow and Research Associate fellowship, respectively. The authors also acknowledge financial support from the European Union’s Horizon 2020 research and innovation program under grant agreement no. 731077 (EU FT-ICR MS project, INFRAIA-02-2017) and from the European Union and Wallonia program FEDER BIOMED HUB Technology Support (No. 2.2.1/996) for the funding of the SolariX XR 9.4T. The authors also thank the European Union’s Horizon 2020 program (EURLipids Interreg Eurogio Meuse-Rhine project supported by the European Regional Development Fund (FEDER)) for the funding of the SunChrom sprayer.
Mice were previously euthanised by cervical dislocation with the approval of the Institutional Animal Ethics Committee of the University of Liege.
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Published in the topical collection Mass Spectrometry Imaging 2.0 with guest editors Shane R. Ellis and Tiffany Porta Siegel.
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Müller, W.H., Verdin, A., Kune, C. et al. Dual-polarity SALDI FT-ICR MS imaging and Kendrick mass defect data filtering for lipid analysis. Anal Bioanal Chem 413, 2821–2830 (2021). https://doi.org/10.1007/s00216-020-03020-w
- Mass spectrometry imaging
- Kendrick mass defect