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A dual-modal colorimetric and photothermal assay for glutathione based on MnO2 nanosheets synthesized with eco-friendly materials

Analytical and Bioanalytical Chemistry volume 412, pages 8443–8450 (2020)Cite this article

Abstract

We developed a dual-modal colorimetric and photothermal assay for glutathione (GSH) using MnO2 nanosheets prepared with environmentally friendly materials. The nanosheets were synthesized by using ascorbic acid present abundantly in lemon and orange juices to reduce KMnO4. The as-prepared MnO2 nanosheets display oxidase-like activity and can catalyze the oxidation reaction of 3,3′,5,5′-tetramethylbenzidine (TMB), yielding a blue oxidative product (oxTMB) that exhibits a UV–Vis absorption peak at 652 nm. In the presence of GSH, the MnO2 nanosheets are reduced and decomposed, resulting in a decrease in the peak intensity. The colorimetric assay offers a wide dynamic range (0.1–100 μM) and a detection limit of 100 nM. The MnO2 nanosheets are also efficient in converting photoenergy to thermal energy, with a photothermal conversion efficiency of 23.3%. The temperature change, after near-infrared (NIR) irradiation at 808 nm, can be easily measured by an inexpensive pen-type thermometer. This effect can also be used for GSH quantification and expands the GSH concentration detection to the range from 6.0 to 200 μM. The viability of our dual-modal assay for clinical applications was demonstrated with successful analyses of GSH in human serum samples.

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Acknowledgments

The authors gratefully acknowledge the TEM analysis provided by Dr. Huailin Fan (University of Jinan) and editorial assistance provided by Dr. Feimeng Zhou (University of Jinan).

Funding

We receive financial support from the Nature Science Foundation of China (Nos. 21906065, 21802052, and 21802051), the Natural Science Foundation of Shandong Province of China (Nos. ZR2019MB066 and ZR2019QB007), and the Shandong Provincial Program of Talent-Leading Teams.

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Affiliations

  1. Institute of Surface Analysis and Chemical Biology, University of Jinan, Jinan, 250022, Shandong, China

    Dandan Liu, Qingqing Tu, Xiaoying Wang, Qing Kang, Pengcheng Wang & Wenjuan Guo

  2. Department of Oncology and Radiotherapy, Shandong Provincial Qianfoshan Hospital, The First Hospital Affiliated with Shandong First Medical University, Jinan, 250014, Shandong, China

    Ying Han

  3. State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Jinan, 250353, Shandong, China

    Xiaoying Wang

Authors
  1. Dandan Liu
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  2. Qingqing Tu
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  3. Ying Han
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  4. Xiaoying Wang
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  5. Qing Kang
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  6. Pengcheng Wang
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  7. Wenjuan Guo
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Corresponding authors

Correspondence to Pengcheng Wang or Wenjuan Guo.

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The current study was in accordance with the 1964 Helsinki declaration and approved by the Medical Ethics Committee of Shandong Provincial Qianfoshan Hospital, affiliated with Shandong First Medical University. Written informed consent was obtained from each participant.

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Liu, D., Tu, Q., Han, Y. et al. A dual-modal colorimetric and photothermal assay for glutathione based on MnO2 nanosheets synthesized with eco-friendly materials. Anal Bioanal Chem 412, 8443–8450 (2020). https://doi.org/10.1007/s00216-020-02982-1

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  • DOI: https://doi.org/10.1007/s00216-020-02982-1

Keywords

  • MnO2 nanosheets
  • Dual-modal assay
  • Colorimetry
  • Photothermal analysis
  • GSH