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Magnetic bead-enzyme assemble for triple-parameter telomerase detection at single-cell level

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Abstract

In this work, we developed a triple-parameter strategy for the detection of telomerase activity from cancer cells and urine samples. This strategy was developed based on magnetic bead-enzyme hybrids combined with fluorescence analysis, colorimetric assay, or adenosine triphosphate (ATP) meter as readout. The application of magnetic bead-enzyme hybrids has the advantages of magnetic separation and signal amplification. These detection methods can be used individually or in combination to achieve the optimal sensing performance and make the results more convincing. Among them, the ATP meter with portable size had easy operation and low cost, and this response strategy provided a higher sensitivity at the single-cell level. The designed strategy was suitable as naked-eye sensor and point-of-care testing (POCT) for rapid assaying of telomerase activity.

Magnetic bead-enzyme assemble for triple-parameter telomerase detection

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Funding

This work was supported by the National Natural Science Foundation of China (21675119, 21705110, and 81772737), the Natural Science Foundation of Guangdong (2018B030306046), the Project Funded by China Postdoctoral Science Foundation (2019M663154), the Shenzhen Municipal Government of China (JCYJ20170413161749433, JSGG20160301161836370), the Sanming Project of Shenzhen Health and Family Planning Commission (SZSM201412018, SZSM201512037), and the High Level University’s Medical Discipline Construction (2016031638).

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Author notes

  1. Yingxin Ma, Guobin Mao and Guoqiang Wu contributed equally to this work.

Authors and Affiliations

  1. Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, Guangdong, China

    Yingxin Ma & Guobin Mao

  2. Key Laboratory of Medical Reprogramming Technology, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen University School of Medicine, Shenzhen, 518039, Guangdong, China

    Guoqiang Wu & Weiren Huang

  3. Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Sciences, Wuhan University, Wuhan, 430072, Hubei, China

    Zhike He

Authors
  1. Yingxin Ma
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  2. Guobin Mao
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  3. Guoqiang Wu
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  4. Zhike He
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  5. Weiren Huang
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Corresponding author

Correspondence to Weiren Huang.

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The work has been approved by the appropriate ethics committee (Medical Ethics Committee of Shenzhen Second People’s Hospital) and has been performed in accordance with the ethical standards.

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The authors declare that they have no conflict of interest.

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Ma, Y., Mao, G., Wu, G. et al. Magnetic bead-enzyme assemble for triple-parameter telomerase detection at single-cell level. Anal Bioanal Chem 412, 5283–5289 (2020). https://doi.org/10.1007/s00216-020-02741-2

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  • DOI: https://doi.org/10.1007/s00216-020-02741-2

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