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Myoglobin-silver reduced graphene oxide nanocomposite stochastic biosensor for the determination of luteinizing hormone and follicle-stimulating hormone from saliva samples

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Abstract

A silver reduced graphene oxide (Ag-rGO) nanocomposite paste was prepared for the assay of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) in children’s saliva. The paste was modified with a solution of myoglobin (Myb) to form the stochastic biosensor, to improve the sensitivity of the method. The Ag-rGO powder and Myb-Ag-rGO paste were morphologically and structurally characterized by SEM, TEM and XRD measurements. For the molecular recognition of the hormones, the biosensor based on Myb-Ag-rGO reached determination limits of 8.5 × 10−3 UI L−1 (2.0 × 10−12 g/mL) for luteinizing hormone and 1.4 × 10−2 UI L−1 (1.0 × 10−12 g/mL) for follicle-stimulating hormone. The determination of both hormones in saliva samples collected from children was done with high reliability.

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Acknowledgments

The authors are grateful to Dr. Lucian-Barbu Tudoran for performing TEM/SEM measurements and to PhD student Alexandru Turza for recording the XRD measurements.

Funding

This work was financially supported by a grant of the Ministry of Research and Innovation, CNCS-UEFISCDI, project number PN-III-P1-1.1-PD-2016-0190, within PNCDI III. TEM/SEM measurements were partially supported through the infrastructure obtained in the Project: Research Center and Advanced Technologies for Alternative Energies - CETATEA - 623/11.03.2014.

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Authors and Affiliations

  1. Laboratory of Electrochemistry and PATLAB, National Institute of Research and Development for Electrochemistry and Condensed Matter, 202 Splaiul Independentei Str., 060021, Bucharest-6, Romania

    Livia Alexandra Dinu Gugoasa, Raluca-Ioana Stefan-van Staden & Jacobus Frederick van Staden

  2. National Institute for Research and Development of Isotopic and Molecular Technologies, 67-103 Donat Street, 400293, Cluj-Napoca, Romania

    Maria Coros & Stela Maria Pruneanu

Authors
  1. Livia Alexandra Dinu Gugoasa
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  2. Raluca-Ioana Stefan-van Staden
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  3. Jacobus Frederick van Staden
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  4. Maria Coros
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  5. Stela Maria Pruneanu
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Corresponding author

Correspondence to Livia Alexandra Dinu Gugoasa.

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Informed consent was obtained from all parents or legal tutors of the patients.

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The authors declare that they have no conflicts of interest.

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Published in the topical collection Euroanalysis XX with guest editor Sibel A. Ozkan.

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Gugoasa, L.A.D., Stefan-van Staden, RI., van Staden, J.F. et al. Myoglobin-silver reduced graphene oxide nanocomposite stochastic biosensor for the determination of luteinizing hormone and follicle-stimulating hormone from saliva samples. Anal Bioanal Chem 412, 5191–5202 (2020). https://doi.org/10.1007/s00216-020-02663-z

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  • DOI: https://doi.org/10.1007/s00216-020-02663-z

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