Rice is the principal food in many countries for billions of people and one of the most consumed cereals in the world. The rice plant has the ability to bioaccumulate essential and toxic trace elements such as arsenic. The toxicity of the elements depends not only on their concentration but also on their chemical form and their bioavailability. The inorganic forms of arsenic are more toxic than the organic forms and the toxicity increases with decreasing oxidation states. The consumers of rice in Europe who are the most exposed to inorganic arsenic are children under three, thorough diet (rice-based food). Recently, the European Commission established the maximum levels of inorganic arsenic in foodstuffs. This regulation establishes a maximum level of inorganic arsenic of 100 μg kg−1 in rice destined for the production of food for infants and young children. In order to know the relation between the As ingested and the arsenic absorbed, studies of bioavailability are necessary. We proposed an in vitro digestion method with dialysis to estimate this relation. Furthermore, a bioavailability study of As species in rice was performed in order to know if a change in As species occurred during the gastrointestinal digestion process. Arsenic species were determined in rice and in the dialysate fraction by high-performance liquid chromatography coupled to inductively coupled plasma mass spectrometry (HPLC-ICP-MS). The proposed method has been applied to different rice samples acquired in the local Spanish market.
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Burlo F, Ramírez-Gandolfo A, Signes-Pastor AJ, Haris PI, Carbonell-Barrachina AA. Arsenic contents in Spanish infant rice, pureed infant foods, and rice. J Food Sci. 2012;77(1):T15–9.
Kim J-Y, Kim W-I, Kunhikrishnan A, Kang D-W, Kim D-H, Lee Y-J, et al. Determination of arsenic species in rice grains using HPLC-ICP-MS. Food Sci Biotechnol. 2013;22(6):1509–13.
Sommella A, Deacon C, Norton G, Pigna M, Violante A, Meharg AA. Total arsenic, inorganic arsenic, and other elements concentrations in Italian rice grain varies with origin and type. Environ Pollut. 2013;181:38–43.
Maher W, Foster S, Krikowa F, Donner E, Lombi E. Measurement of Inorganic Arsenic Species in Rice after Nitric Acid Extraction by HPLC-ICPMS: Verification Using XANES. Environ Sci Technol. 2013;47(11):5821–7.
Halder D, Biswas A, Šlejkovec Z, Chatterjee D, Nriagu J, Jacks G, et al. Arsenic species in raw and cooked rice: Implications for human health in rural Bengal. Sci Total Environ. 2014;497:200–8.
Rahman MA, Rahman MM, Reichman SM, Lim RP, Naidu R. Arsenic Speciation in Australian-Grown and Imported Rice on Sale in Australia: Implications for Human Health Risk. J Agric Food Chem. 2014;62(25):6016–24.
Lin K, Lu S, Wang J, Yang Y. The arsenic contamination of rice in Guangdong Province, the most economically dynamic provinces of China: arsenic speciation and its potential health risk. Environ Geochem Health. 2015;37(2):353–61.
Tenni D, Martin M, Barberis E, Beone GM, Miniotti E, Sodano M, et al. Total As and As Speciation in Italian Rice as Related to Producing Areas and Paddy Soils Properties. J Agric Food Chem. 2017;65(17):3443–52.
Adeyemi JA, Adedire CO, Martins AD, Paulelli AC, Awopetu AF, Segura FR, et al. Arsenic speciation in rice consumed in south-western Nigeria, and estimation of dietary intake of arsenic species through rice consumption. Toxicol Environ Chem. 2017;99(5–6):999–1006.
Farías SS, Londonio A, Quintero C, Befani R, Soro M, Smichowski P. On-line speciation and quantification of four arsenical species in rice samples collected in Argentina using a HPLC-HG-AFS coupling. Microchem J. 2015;120:34–9.
Segura FR, de Oliveira JM, Silva E, da Cunha A, Cavalheiro AC, Barbosa F, et al. Arsenic speciation in Brazilian rice grains organically and traditionally cultivated: is there any difference in arsenic content? Food Res Int. 2016;89:169–76.
Sharma VK, Sohn M. Aquatic arsenic: toxicity, speciation, transformations, and remediation. Environ Int. 2009;35:743–59.
IARC (International Agency for Research Cancer). IARC Monographs on the evaluation of Carcinogenic Risks to Humans. Overall evaluation of carcinogenic: an updating of IARC Monographs; IARC: Lyon 1987;1(suppl. 7):1–42.
WHO. Evaluation of Certain Food Additives and Contaminants, 33rd Report of the Joint FAO/WHO Expert Committee on Food Additives; Technical Report Series 776; WHO: Geneva, 1989.
Meharg AA, Williams PN, Deacon CM, Norton GJ, Hossain M, Louhing D, et al. Urinary excretion of arsenic following rice consumption. Environ Pollut. 2014;194:181–7.
Juhasz AL, Smith E, Weber J, Rees M, Rofe A, Kuchel T, et al. In vivo assessment of arsenic bioavailability in rice and its significance for human health risk assessment. Environ Health Perspect. 2006;114(12):1826–31.
Islam S, Rahman MM, Duan L, Islam MR, Kuchel T, Naidu R. Variation in arsenic bioavailability in rice genotypes using swine model: an animal study. Sci Total Environ. 2017;599:324–31.
Li HB, Li J, Zhao D, Li C, Wang X, Sun H, et al. Arsenic relative bioavailability in rice using a mouse arsenic urinary excretion bioassay and its application to assess human health risk. Environ Sci Technol. 2017;51(8):4689–96.
Rees M, Sansom L, Rofe A, Juhasz AL, Smith E, Weber J, et al. Principles and application of an in vivo swine assay for the determination of arsenic bioavailability in contaminated matrices. Environ Geochem Health. 2009;31:167–77.
Horner NS, Beauchemin D. A simple method using on-line continuous leaching and ion exchange chromatography coupled to inductively coupled plasma mass spectrometry for the speciation analysis of bio-accessible arsenic in rice. Anal Chim Acta. 2012;717:1–6.
He Y, Pedigo CE, Lam B, Cheng Z, Zheng Y. Bioaccessibility of arsenic in various types of rice in an in vitro gastrointestinal fluid system. J Environ Sci Health B. 2012;47(2):74–80.
Rosas-Castor JM, Portugal L, Ferrer L, Hinojosa-Reyes L, Guzmán-Mar JL, Hernández-Ramírez A, et al. An evaluation of the bioaccessibility of arsenic in corn and rice samples based on cloud point extraction and hydride generation coupled to atomic fluorescence spectrometry. Food Chem. 2016;204:475–82.
Sun GX, Van de Wiele T, Alava P, Tack F, Laing GD. Arsenic in cooked rice: effect of chemical, enzymatic and microbial processes on bioaccessibility and speciation in the human gastrointestinal tract. Environ Pollut. 2012;162:241–6.
Laparra JM, Vélez D, Barberá R, Farré R, Montoro R. Bioavailability of inorganic arsenic in cooked rice: practical aspects for human health risk assessments. J Agric Food Chem. 2005;53(22):8829–33.
Lee SG, Kim J, Park H, Holzapfel W, Lee KW. Assessment of the effect of cooking on speciation and bioaccessibility/cellular uptake of arsenic in rice, using in vitro digestion and Caco-2 and PSI cells as model. Food Chem Toxicol. 2018;111:597–604.
Domínguez-González MR, Romarís-Hortas V, García-Sartal C, Moreda-Piñeiro A, Barciela-Alonso MC, Bermejo-Barrera P. Evaluation of an in vitro method to estimate trace elements bioavailability in edible seaweeds. Talanta. 2010;82:1668–76.
García-Sartal C, Romarís-Hortas V, Barciela-Alonso MC, Moreda-Piñeiro A, Domínguez-González MR, Bermejo-Barrera P. Use of an in-vitro digestion method to evaluate the bioaccessibility of arsenic in edible seaweed by inductively coupled plasma-mass spectrometry. Microchem J. 2011;98:91–6.
Moreda-Piñeiro J, Herbello-Hermelo P, Domínguez-González MR, Bermejo-Barrera P, Moreda-Piñeiro A. Bioavailability assessment of essential and toxic metals in edible nuts and seeds. Food Chem. 2016;205:146–54.
Latorre M, Peña-Farfal C, Neira Y, Herbello-Hermelo P, Domínguez-González MR, Bermejo-Barrera P, et al. In vitro human bioavailability of major, trace and ultra-trace elements in Chilean “natural” wines from Itata Valley. Food Funct. 2018;9:5381–9.
Batista BL, Souza JMO, De Souza SS, Barbosa F Jr. Speciation of arsenic in rice and estimation of daily intake of different arsenic species by Brazilians through rice consumption. J Hazard Mater. 2011;191(1–3):342–8.
Sumontha N, Nuchanart R, Chulabhorn M, Gunlatida P, Jutamaad S. Determination of arsenic species in rice from Thailand and other Asian countries using simple extraction and HPLC-ICP-MS analysis. J Agric Food Chem. 2013;61(28):6991–8.
This program received financial support from Xunta de Galicia (Grupo de Referencia Competitiva ED431C2018/19). This program is co-funded by FEDER (UE).
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Domínguez-González, M.R., Barciela-Alonso, M., Calvo-Millán, V.G. et al. The bioavailability of arsenic species in rice. Anal Bioanal Chem 412, 3253–3259 (2020). https://doi.org/10.1007/s00216-020-02589-6