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Multi-technique microscopy investigation on bacterial biofilm matrices: a study on Klebsiella pneumoniae clinical strains

Abstract

Biofilms are communities of bacteria living embedded in a highly hydrated matrix composed of polysaccharides, proteins, and extracellular DNA. This life style confers numerous advantages to bacteria including protection against external threats. However, they also contribute to increase bacterial resistance against antimicrobials, an issue particularly relevant in dangerous infections. Due to the complexity of the matrix, few information is present in the literature on details of its architecture including the spatial distribution of the macromolecular components which might give hints on the way the biofilm scaffold is built up by bacteria. In this study, we investigated the possibility to combine well-established microbiological procedures with advanced microscopies to get information on composition and distribution of the macromolecular components of biofilm matrices. To this, confocal microscopy, diffraction-limited infrared (IR) spectral imaging, and atomic force microscopy (AFM) were used to explore biofilm produced by a clinical strain of Klebsiella pneumoniae. IR imaging permitted to have clues on how the biofilm grows and spreads on surfaces, and the local distribution of the components within it. Through the analysis of the pure component spectra, it was possible to assess the chemical and structural composition of the saccaridic matrix, confirming the data obtained by NMR. It was also possible to follow the time course of biofilm from 6 up to 48 h when the biofilm grew into a 3-dimensional multi-layered structure, characteristic of colonies of bacteria linked together by a complex matrix. In addition, nanoFTIR and AFM investigations allowed the estimation of biofilm growth in the vertical direction and the morphological analysis of bacterial colonies at different time points and the evaluation of the chemical composition at the nanoscale.

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References

  1. 1.

    Flemming H-C, Wingender J, Szewzyk U, Steinberg P, Rice SA, Kjelleberg S. Biofilms: an emergent form of bacterial life. Nat Rev Microbiol. 2016;14:563.

    CAS  Article  Google Scholar 

  2. 2.

    Costerton JW, Lewandowski Z, Caldwell DE, Korber DR, Lappin-Scott HM. Microbial biofilms. Annu Rev Microbiol. 1995;49:711.

    CAS  Article  Google Scholar 

  3. 3.

    Branda SS, Vik Å, Friedman L, Kolter R. Biofilms: the matrix revisited. Trends Microbiol. 2005;13:20.

    CAS  Article  Google Scholar 

  4. 4.

    Flemming H-C, Neu T, Wozniak DJ. The EPS matrix: the “house of biofilm cells”. J Bacteriol. 2007;189:7945.

    CAS  Article  Google Scholar 

  5. 5.

    Fux CA, Costerton JW, Stewart PS, Stoodley P. Survival strategies of infectious biofilms. Trends Microbiol. 2005;13:34.

    CAS  Article  Google Scholar 

  6. 6.

    Birarda G, Holman EA, Fu S, Weikel K, Hu P, Blankenberg FG, et al. Synchrotron infrared imaging of advanced glycation endproducts (AGEs) in cardiac tissue from mice fed high glycemic diets. Biomed Spectrosc Imaging. 2013;2:301.

    CAS  PubMed  PubMed Central  Google Scholar 

  7. 7.

    Li B, Zhao Y, Liu C, Chen Z, Zhou D. Molecular pathogenesis of Klebsiella pneumoniae. Future Microbiol. 2014;9:1071.

    Article  Google Scholar 

  8. 8.

    Munoz-Price LS, Poirel L, Bonomo RA, Schwaber MJ, Daikos GL, Cormican M, et al. Clinical epidemiology of the global expansion of Klebsiella pneumoniae carbapenemases. Lancet Infect Dis. 2013;13:785.

    Article  Google Scholar 

  9. 9.

    Cescutti P, De Benedetto G, Rizzo R. Structural determination of the polysaccharide isolated from biofilms produced by a clinical strain of Klebsiella pneumoniae. Carbohydr Res. 2016;430:29.

    CAS  Article  Google Scholar 

  10. 10.

    Benincasa M, Lagatolla C, Dolzani L, Milan A, Pacor S, Liut G, et al. Biofilms from Klebsiella pneumoniae: matrix polysaccharide structure and interactions with antimicrobial peptides. Microorganisms. 2016;4:26.

    Article  Google Scholar 

  11. 11.

    Whitfield C, Richards JC, Perry MB, Clarke BR, MacLean LL. Expression of two structurally distinct D-galactan O antigens in the lipopolysaccharide of Klebsiella pneumoniae serotype O1. J Bacteriol. 1991;173:1420.

    CAS  Article  Google Scholar 

  12. 12.

    Merritt JH, Kadouri DE, O’Toole GA. Growing and analyzing static biofilms. Curr Protoc Microbiol. 2005 Jul;Chapter 1:Unit 1B.1.

  13. 13.

    Heydorn A, Nielsen AT, Hentzer M, Sternberg C, Givskov M, Ersbøll BK, et al. Quantification of biofilm structures by the novel computer program COMSTAT. Microbiology. 2000;146:2395.

    CAS  Article  Google Scholar 

  14. 14.

    Vorregaard M. Comstat2 - a modern 3D image analysis environment for biofilms. Master thesis, Technical University of Denmark (DTU), 2008.

  15. 15.

    Lupi S, Nucara A, Perucchi A, Calvani P, Ortolani M, Quaroni L, et al. Performance of SISSI, the infrared beamline of the ELETTRA storage ring. J Opt Soc Am B. 2007;24:959.

    CAS  Article  Google Scholar 

  16. 16.

    Armitano J, Mejean V, Jourlin-Castelli C. Gram-negative bacteria can also form pellicles. Environ Microbiol Rep. 2014;6:534.

    Article  Google Scholar 

  17. 17.

    Lewis AT, Jones K, Lewis KE, Jones S, Lewis PD. Detection of Lewis antigen structural change by FTIR spectroscopy. Carbohydr Polym. 2013;92:1294–301.

    CAS  Article  Google Scholar 

  18. 18.

    Socrates G. Infrared and Raman characteristic group frequencies: tables and charts. 3rd ed. Chichester: Wiley; 2004.

    Google Scholar 

  19. 19.

    Probst AJ, Holman HY, DeSantis TZ, Andersen GL, Birarda G, Bechtel HA, et al. Tackling the minority: sulfate-reducing bacteria in an archaea-dominated subsurface biofilm. ISME J. 2013;7:635.

    CAS  Article  Google Scholar 

  20. 20.

    Schlafer S, Meyer RL. Confocal microscopy imaging of the biofilm matrix. J Microbiol Methods. 2017;138:50.

    Article  Google Scholar 

  21. 21.

    Mandal DK, Bhattacharyya L, Koenig SH, Brown RD, Oscarson S, Brewer CF. Studies of the binding specificity of concanavalin A. Nature of the extended binding site for asparagine-linked carbohydrates. Biochemistry. 1994;33:1157.

    CAS  Article  Google Scholar 

  22. 22.

    Kaewpijit S, Le Moigne J, El-Ghazawi T. Automatic reduction of hyperspectral imagery using wavelet spectral analysis. IEEE Geosci Remote Sensing. 2003;41:863.

    Article  Google Scholar 

  23. 23.

    Roggo Y, Edmond A, Chalus P, Ulmschneider M. Infrared hyperspectral imaging for qualitative analysis of pharmaceutical solid forms. Anal Chim Acta. 2005;535:79.

    CAS  Article  Google Scholar 

  24. 24.

    Neu TR, Manz B, Volke F, Dynes JJ, Hitchcock AP, Lawrence JR. Advanced imaging techniques for assessment of structure, composition and function in biofilm systems. FEMS Microbiol Ecol. 2010;72(1):1–21.

    CAS  Article  Google Scholar 

  25. 25.

    Neu TR, Swerhone GDW, Lawrence JR. Assessment of lectin-binding analysis for in situ detection of glycoconjugates in biofilm systems. Microbiology. 2001;147:299.

    CAS  Article  Google Scholar 

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Acknowledgements

We thank Dr. Philip Schäfer from NEASPEC GmbH for the support during these first measurements.

Funding

This study was funded by the University of Trieste (FRA 13 and FRA15). Images in this paper were generated at the Light Microscopy Imaging Centre (LMIC) of the University of Trieste - Life Sciences Department, funded as detailed at www.units.it/confocal.

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Correspondence to Roberto Rizzo.

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Birarda, G., Delneri, A., Lagatolla, C. et al. Multi-technique microscopy investigation on bacterial biofilm matrices: a study on Klebsiella pneumoniae clinical strains. Anal Bioanal Chem 411, 7315–7325 (2019). https://doi.org/10.1007/s00216-019-02111-7

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Keywords

  • AFM (atomic force microscopy)
  • NanoFTIR
  • Biofilm
  • Multi-variate analysis
  • IR spectroscopy