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Self-heating tilted fiber Bragg grating device for melt curve analysis of solid-phase DNA hybridization and thermal cycling

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Abstract

We demonstrate a DNA-based optical fiber device that uses an in-fiber grating, a light absorbing coating with surface anchored DNA, and a built-in optical thermometer. This device is used for precisely thermal cycling surface DNA spots bound by a simple UV cross-linking technique. Near-infrared light of wavelengths near 1550 nm and guided power near 300 mW is coupled out of the fiber core by a tilted fiber Bragg grating inscribed in the fiber and absorbed by the coating to increase its temperature to more than 95 °C. A co-propagating broadband light signal (also in the near-infrared region) is used to measure the reflection spectrum of the grating and thus the temperature from the wavelength shifts of the reflection peaks. The device is capable of sensitive DNA melt analysis and can be used for DNA amplification.

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Acknowledgements

This work was funded by grants from the Natural Sciences and Engineering Research Council of Canada and Spartan Biosciences Inc. Gratings were fabricated in the Advanced Photonics Laboratory of Carleton University by Albane Laronche.

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Authors and Affiliations

  1. Department of Electronics, Carleton University, 1125 Colonel By Drive, Ottawa, ON, K1S 5B6, Canada

    Jason Koppert, Hubert Jean-Ruel & Jacques Albert

  2. Spartan Biosciences Inc., 2934 Baseline Road #500, Nepean, ON, K2H B2, Canada

    Hubert Jean-Ruel & Chris Harder

  3. Department of Chemistry, Carleton University, 1125 Colonel By Drive, Ottawa, ON, K1S 5B6, Canada

    Devin O’Neill & Anatoli Ianoul

  4. Department of Biochemistry, Carleton University, 1125 Colonel By Drive, Ottawa, ON, K1S 5B6, Canada

    William Willmore

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  1. Jason Koppert
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  2. Hubert Jean-Ruel
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  3. Devin O’Neill
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  4. Chris Harder
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  5. William Willmore
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  6. Anatoli Ianoul
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  7. Jacques Albert
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Correspondence to Jacques Albert.

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The authors declare that they have no competing interests.

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Koppert, J., Jean-Ruel, H., O’Neill, D. et al. Self-heating tilted fiber Bragg grating device for melt curve analysis of solid-phase DNA hybridization and thermal cycling. Anal Bioanal Chem 411, 6813–6823 (2019). https://doi.org/10.1007/s00216-019-02072-x

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  • DOI: https://doi.org/10.1007/s00216-019-02072-x

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