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Optical biosensing of Streptococcus agalactiae based on core/shell magnetic nanoparticle-quantum dot

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Abstract

An immunomagnetic optical probe based on a core/shell magnetic nanoparticle–quantum dot was fabricated for detection of Streptococcus agalactiae, the causative agent of pneumonia and meningitis in newborns. The silica-coated magnetic nanoparticles conjugated with anti-S. agalactiae monoclonal antibody provided high specificity for pre-enrichment of bacteria from biological samples with a complex matrix such as milk. Compared with conventional methods such as culture and molecular techniques, the combination of fluorescent quantum dot and magnetic nanoparticle enhanced the sensitivity and speed of bacterial identification. The bio-functionalized fluorescent-magnetic nanoparticles were characterized by TEM, SEM, VSM, XRD, DLS, and FTIR and applied to the detection of S. agalactiae with a limit of 10 and 102 CFU/mL in PBS and milk, respectively. This immunomagnetic optical probe can be used for rapid isolation, sensitive, and specific detection of targeted bacteria without any treatment in clinical and animal samples in the presence of other infectious agents.

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Abbreviations

Ab:

Antibody

FMNP-Ab:

Antibody-conjugated FMNPs

FMNPs:

Fluorescent-magnetic nanocomposite

MNPs:

Magnetic nanoparticles

QDs:

Quantum dots

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Acknowledgment

We are grateful for the financial support from the Iran National Institute for Medical Research Development (NIMAD) (Grant No. 940990).

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Authors and Affiliations

  1. Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, 14115-154, Iran

    Razieh Ghasemi & Mehrdad Behmanesh

  2. Department of Molecular Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, 81651-31378, Iran

    Razieh Ghasemi, Seyede Zohreh Mirahmadi-zare & Mohammad Hossein Nasr-Esfahani

  3. Research Institute for Nanotechnology and Advanced Materials, Isfahan University of Technology, Isfahan, 84156-83111, Iran

    Alireza Allafchian

  4. Department of Genetic, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, 14115-154, Iran

    Mehrdad Behmanesh

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  1. Razieh Ghasemi
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  2. Seyede Zohreh Mirahmadi-zare
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  3. Mohammad Hossein Nasr-Esfahani
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Correspondence to Seyede Zohreh Mirahmadi-zare or Mehrdad Behmanesh.

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Ghasemi, R., Mirahmadi-zare, S.Z., Nasr-Esfahani, M.H. et al. Optical biosensing of Streptococcus agalactiae based on core/shell magnetic nanoparticle-quantum dot. Anal Bioanal Chem 411, 6733–6743 (2019). https://doi.org/10.1007/s00216-019-02046-z

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  • DOI: https://doi.org/10.1007/s00216-019-02046-z

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